| Field | Specification |
|---|---|
| Target | |
| Alternative names | Platelet endothelial aggregation receptor 1; hPEAR1; Multiple epidermal growth factor-like domains protein 12; Multiple EGF-like domains protein 12; PEAR1; MEGF12 |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: CHO; Immunogen sequence: L21-S754 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human platelet endothelial aggregation receptor 1 (gene PEAR1) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
PEAR1 (platelet endothelial aggregation receptor 1) is a transmembrane receptor with multiple EGF-like repeats expressed on platelets and endothelial cells. It supports platelet activation, for example in response to its ligand SVEP1 through AKT/mTOR signaling, and PEAR1 is studied in platelet function, thrombosis and endothelial biology.
Kit Components
| Anti-Human PEAR1 Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Human PEAR1 Standard | 2 × 20 ng/tube |
| Human PEAR1 Biotinylated Antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Plate Sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <150 pg/mL |
| Standard | Recombinant human PEAR1 (Leu21–Ser754), expressed in CHO |
| Cross-reactivity | No significant cross-reactivity or interference with PEAR1 analogs in supplier testing; untested analogs cannot be excluded. |
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.015 | 0.09 | 0.152 | 0.288 | 0.55 | 0.927 | 1.539 | 2.114 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 526 | 1057 | 7376 | 503 | 771 | 6486 |
| Standard deviation | 34.51 | 37.1 | 478.7 | 16.05 | 60.75 | 323.65 |
| CV(%) | 6.6% | 6.5% | 6.5% | 3.2% | 7.9% | 5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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