| Field | Specification |
|---|---|
| Target | |
| Alternative names | HARP; HBGF8; HBNF; NEGF1; heparin affin regulatory protein|heparin binding growth factor 8|heparin-binding growth-associated molecule|neurite growth-promoting factor 1|pleiotrophin (heparin binding |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Pleiotrophin was initially recognized as a neurite outgrowth-promoting factor present in rat brain around birth and as a mitogen toward fibroblasts isolated from bovine uterus tissue. Together with midkine these growth-factors constitute a family of secreted heparin-binding proteins now known as the Neurite Growth-promoting Factor (NEGF) family. During embryonic and early postnatal development, Pleiotrophin is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. Pleiotrophin is also expressed by several tumor cells and is thought to be involved in tumor angiogenesis. In the adult central nervous system. Pleiotrophin is expressed in an activity-dependent manner in the hippocampus, where it can suppress long term potentiation induction.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate PTN in samples. An antibody specific for PTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 31.25-2000 pg/mL |
| Sensitivity | 11.7 pg/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE63018HU |
| UniProt # | P21246 |
| Alternative Names | HARP; HBGF8; HBNF; NEGF1; heparin affin regulatory protein|heparin binding growth factor 8|heparin-binding growth-associated molecule|neurite growth-promoting factor 1|pleiotrophin (heparin binding |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for PTN research.
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