| Field | Specification |
|---|---|
| Target | |
| Alternative names | DKFZp686F0937; FLJ36270; FLJ45632; TEM3; TEM7; 2410003I07Rik|tumor endothelial marker 7 |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
TEM7 was expressed clearly in the endothelial cells of the tumor stroma but not in the endothelial cells of normal colonic tissue. Using in situ hybridization to assay expression in various normal adult mouse tissues, they observed that Tem7 was largely undetectable in mouse tissues or tumors, but was abundantly expressed in mouse brain. TEM7 coding region, which encodes a 500-amino acid type I transmembrane protein containing a plexin-like domain. TEM3 and TEM7 differ in the use of alternative polyadenylation sites but result in the same predicted protein. The mouse ortholog of TEM7 shares 81% amino acid identity with the human protein.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate PLXDC1 in samples. An antibody specific for PLXDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 62.5-4000 pg/mL |
| Sensitivity | 38 pg/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE58045HU |
| UniProt # | Q8IUK5 |
| Alternative Names | DKFZp686F0937; FLJ36270; FLJ45632; TEM3; TEM7; 2410003I07Rik|tumor endothelial marker 7 |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for PLXDC1 research.
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