| Field | Specification |
|---|---|
| Target | |
| Alternative names | RP11-125A15.2; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|poly (ADP-ribose) polymerase family; member 1|po |
| UniProt # | |
| Species | |
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| Assay type | |
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Scientific Background
PARP is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress . This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo . In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) . PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate PARP1 in samples. An antibody specific for PARP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 0.78-50 ng/mL |
| Sensitivity | 0.28 ng/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE28636HU |
| UniProt # | P09874 |
| Alternative Names | RP11-125A15.2; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)|ADP-ribosyltransferase NAD(+)|poly (ADP-ribose) polymerase family; member 1|po |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for PARP1 research.
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