| Field | Specification |
|---|---|
| Mfr No | |
| Product Format | Frozen |
| Product Type | |
| Shipping | |
| Storage |
Overview
Fully Characterized Human Preadipocytes, Subcutaneous, Obese Adult (HPA-o1)These primary human preadipocytes are isolated from subcutaneous adipose tissue of an obese adult donor and fully characterized for research applications. They retain key preadipocyte markers, differentiation capacity, and functional properties, providing a reliable in vitro model for adipogenesis, obesity research, metabolic studies, and lipid metabolism. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Adipose
- Growth properties: Adherent, fibroblast-like
- Format: Frozen
- Donor history: Female, 58, Caucasian, BMI 30.4
- Biosafety level: II
- Culture context: Cell Expansion and Maintenance:PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.
Adipose-lineage cells are used to study differentiation, lipid handling, endocrine signaling, and inflammatory remodeling in adipose tissue models.
Research relevance and current trends
- Primary adipose-lineage cells are used to compare donor-specific adipogenic capacity and metabolic responsiveness.
- Researchers frequently assess inflammatory remodeling and adipokine-associated signaling in differentiated cultures.
- Differentiation-state tracking remains central to adipose tissue modeling and screening workflows.
Common research applications
- Monitor adipogenic differentiation and lipid-associated marker changes over time.
- Study metabolic or inflammatory responses in adipose-relevant culture systems.
- Use in conditioned-media or co-culture assays to probe adipose crosstalk.
Product-specific data supplied for this listing
- Growth Conditions: Cell Expansion and Maintenance:PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. Preadipocyte Medium Kit (TM048) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Adipocyte Differentiation:PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
