| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human Primary CD34+ Blood Cells are hematopoietic stem and progenitor cells isolated from healthy adult donors. These cells provide a physiologically relevant in vitro model for studying hematopoiesis, blood cell differentiation, gene therapy, and immune system function. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Blood (Immune)
- Growth properties: Suspension, round
- Format: Frozen
- Seeding guidance: 5000 cells/ml
- Biosafety level: II
- Culture context: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) .
Primary cells provide a biologically relevant starting point for in vitro studies because they retain tissue-linked morphology, growth behavior, and donor-associated characteristics that are often reduced in immortalized systems.
Research relevance and current trends
- Primary-cell models continue to be used when donor context, tissue specificity, and physiologic responsiveness are important.
- Researchers often combine primary cells with co-culture, matrix, or conditioned-media approaches to better reflect native microenvironments.
- Phenotypic characterization and careful tracking of culture conditions remain important for reproducible interpretation.
Common research applications
- Use in tissue-relevant in vitro assays where morphology and donor context matter.
- Measure phenotype, marker expression, or response to defined perturbations over time.
- Incorporate into co-culture or screening workflows requiring primary-cell context.
Product-specific data supplied for this listing
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . PriGrow X Series Medium (TM5402) + TM5402-CM + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: TM5402 must be supplemented with TM5402-CM (cytokine mix) for successful cell expansion. TM5402-CM is available as aliquots for either 100ml or 500ml of complete media.
- Split Ratio: 1:3 to 1:8
- Seeding Density (cells/ml): 5000
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 240xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions for 2-3 days.
- Add more fresh media daily, during this time; do not perform a media change. Expand Cells (3+ Days)
- Collect cells and centrifuge at 240xg for 10 minutes to form a cell pellet.
- Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
