| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human Primary Kidney Glomerular Endothelial Cells are human frozen primary cells from kidney used for vascular biology, barrier function, leukocyte trafficking, and tissue-specific microvascular studies. These cells display adherent, cobble-stone growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Kidney
- Growth properties: Adherent, cobble-stone
- Format: Frozen
- Reported expression/markers: CD31, VE-Cadherin
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Endothelial Cell Growth Medium Kit (TM104) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
Endothelial cells line the luminal surface of blood vessels and regulate barrier properties, hemostatic balance, immune cell trafficking, angiogenic signaling, and tissue-specific exchange processes.
Research relevance and current trends
- Primary endothelial models are used to study barrier integrity, inflammatory activation, and permeability changes in tissue-specific vascular beds.
- Co-culture systems with immune, stromal, or parenchymal cells are increasingly used to model microenvironment-dependent signaling.
- Researchers often compare donor, vessel-bed, or organ-specific phenotypes to capture biologically relevant heterogeneity.
Common research applications
- Assess barrier function, adhesion molecule regulation, or cytokine-driven endothelial activation in vitro.
- Model angiogenic responses, matrix interactions, and vessel-bed-specific signaling under defined culture conditions.
- Use in co-culture or transwell systems to study leukocyte transmigration and paracrine crosstalk.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Endothelial Cell Growth Medium Kit (TM104) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Disclaimer: 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Marker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.
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🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 8-10 ml of pre-warmed, complete growth media.
- Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge. Centrifuge cells at 120 x g for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 6 ml of recommended pre-warmed, complete growth media and dispense into a gelatin coated T25 culture flask.
- Incubate the cells at the recommended conditions.
- Change media the following day to remove non-adherent cells and replenish nutrients.
- Change cell culture medium every day when cells are >70% confluent. Check cells daily under a microscope to verify appropriate cell morphology.
🔬 Subculture Protocol
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.
- Aspirate the culture media, and perform a wash with sterile 1X PBS (without calcium and magnesium) to dislodge loosely attached cells and remove fraction. Discard the wash solution.
- Incubate cells with 3 ml of pre-warmed (37°C) 0.05% Trypsin-EDTA for 2-5 minutes.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes, may require firm tap if the flasks to disloge cells).
- Once cells detatch add 8-10 ml of complete media with 5-10% FBS to neutralize trypsin immediately. Do not centrifuge the cells.
- Plate cells in fresh flasks or plates pre-coated with gelatin. The recommended split ratio is 1:2.
- Incubate the cells at the recommended conditions.
- Change media the following day to remove non-adherent cells and replenish nutrients.
- Change cell culture medium every 1-2 days or every day when cells are >70% confluent. Pre-wash cells with 1X PBS (with calcium and magenesium) whenever replacing media.
- Check cells daily under a microscope to verify appropriate cell morphology.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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