| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | Normal tissue |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human Primary Skeletal Muscle Cells are human frozen primary cells from skeletal muscle used for tissue-relevant cell biology, phenotypic characterization, and in vitro assay development. These cells display adherent, multipolar growth characteristics.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Skeletal Muscle
- Growth properties: Adherent, multipolar
- Format: Frozen
- Seeding guidance: 3,500 cells/cm²
- Biosafety level: II
- Culture context: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422).
Primary cells provide a biologically relevant starting point for in vitro studies because they retain tissue-linked morphology, growth behavior, and donor-associated characteristics that are often reduced in immortalized systems.
Research relevance and current trends
- Primary-cell models continue to be used when donor context, tissue specificity, and physiologic responsiveness are important.
- Researchers often combine primary cells with co-culture, matrix, or conditioned-media approaches to better reflect native microenvironments.
- Phenotypic characterization and careful tracking of culture conditions remain important for reproducible interpretation.
Common research applications
- Use in tissue-relevant in vitro assays where morphology and donor context matter.
- Measure phenotype, marker expression, or response to defined perturbations over time.
- Incorporate into co-culture or screening workflows requiring primary-cell context.
Product-specific data supplied for this listing
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow X Series Medium (TM4069) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: cells can be maintained in culture for up to 7 days.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Seeding Density (cells/cm²): 3,500
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
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🧊 Thawing Protocol
- Prepare desired culture vessel(s) as per recommended seeding density by adding the appropriate volume of complete growth media. Allow vessel(s) to equilibate to the recommened conditions (37.0°C, 5% CO₂) for at least 30 minutes.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into the culture vessels prepared in Step
- Ensure cells are evenly distributed within the culture flask.
- Incubate the cells at the recommended conditions.
- Do not centrifuge cells to remove DMSO as this action will damage cells.
- Change the media with fresh complete growth media after 24 hours.
🔬 Subculture Protocol
Note: cells have a finite lifespan in vitro and can be propagated for up to 10 population doublings. Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 50-70% confluent and contains many mitotic figures.
- Pre-warm all reagents to room temperature. Prepare culture vessel(s) by adding the required amount of complete growth media and allowing vessel(s) to equilbrate to the recommended conditions (37.0°C, 5% CO₂) for at least 30 minutes.
- Aspirate the culture media, and rinse the cells with 5-10 ml of HEPES-BSS.
- Add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-6 minutes). Cells that are difficult to detach can be incubated at 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 220xg for 5 minutes.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Perform a cell count and add appropriate aliquots of the cell suspension to the prepared culture vessels, as per the recommended seeding density.
- Incubate the cells at the recommended conditions.
- Change growth media every other day, thereafter.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
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