| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Disease | Normal tissue |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human Primary Umbilical Vein Endothelial Cells (HUVEC) are derived from healthy human umbilical vein tissue. These primary HUVECs are ideal for vascular biology research, angiogenesis studies, endothelial function assays, and more. The product is supplied as frozen cells.
Key elements and design rationale
- Biological source: Human (H. sapiens)
- Tissue origin: Placenta
- Growth properties: Adherent, polygonal
- Format: Frozen
- Donor history: Normal tissue
- Biosafety level: II
- Culture context: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth.
Endothelial cells line the luminal surface of blood vessels and regulate barrier properties, hemostatic balance, immune cell trafficking, angiogenic signaling, and tissue-specific exchange processes.
Research relevance and current trends
- Primary endothelial models are used to study barrier integrity, inflammatory activation, and permeability changes in tissue-specific vascular beds.
- Co-culture systems with immune, stromal, or parenchymal cells are increasingly used to model microenvironment-dependent signaling.
- Researchers often compare donor, vessel-bed, or organ-specific phenotypes to capture biologically relevant heterogeneity.
Common research applications
- Assess barrier function, adhesion molecule regulation, or cytokine-driven endothelial activation in vitro.
- Model angiogenic responses, matrix interactions, and vessel-bed-specific signaling under defined culture conditions.
- Use in co-culture or transwell systems to study leukocyte transmigration and paracrine crosstalk.
Product-specific data supplied for this listing
- Growth Conditions: PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. Human Endothelial Cell Growth Medium Kit (TM104) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Cells can only propagate up to 2 passages at split ratio of 1:2. Recommend to conduct media change every 3 days to maintain optimal growth.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Notes for experimental interpretation
- Primary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.
- Attachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.
- Cryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
