| Field | Specification |
|---|---|
| Target | |
| Alternative names | MGC29671; spinster homolog 3 |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
By searching a human database for sequences similar to the SLC22 family of anion transporters , Jacobsson et al. (2007) identified SPNS3. The deduced 512-amino acid protein has 12 putative transmembrane domains. Quantitative real-time PCR detected high expression of rat Spns3 in coronal brain sections of the olfactory bulb and frontal cortex. Expression was moderate in other coronal sections and in thymus, kidney, and spleen. Little to no expression was detected in other tissues examined. Jacobsson et al. (2007) determined that the SPNS3 gene contains 12 exons.Jacobsson et al. (2007) stated that the SPNS3 gene maps to chromosome 17p13.2; the mouse Spns3 gene maps to chromosome 11qB4.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate SPNS3 in samples. An antibody specific for SPNS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPNS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | Available on request — contact us for lot-specific performance data |
| Sensitivity | Available on request — contact us for lot-specific performance data |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE14995HU |
| UniProt # | Q6ZMD2 |
| Alternative Names | MGC29671; spinster homolog 3 |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for SPNS3 research.
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