| Field | Specification |
|---|---|
| Species | |
| Applications | |
| Cell type | |
| Classification | |
| Tissue | |
| Disease | Idiopathic Pulmonary Fibrosis (IPF) |
| Biosafety level | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
Human Pulmonary Fibroblasts (HPF) isolated from lung tissue of patients with idiopathic pulmonary fibrosis (IPF). Fibroblasts play an important role in maintaining the structural integrity of connective tissue and in the synthesis of extracellular matrix proteins; in IPF this programme becomes dysregulated. Disease-derived fibroblasts provide a robust model for in vitro studies of IPF and related fibroblast diseases.
Specifications
| Species | Human (Homo sapiens) |
|---|---|
| Tissue | Lung |
| Disease State | Idiopathic pulmonary fibrosis (IPF) |
| Cell Type | Fibroblasts |
| Product Format | Cryopreserved |
| Pack Size | 0.5 x 106 cells/vial |
| Passage at Cryopreservation | P2 |
| Growth Properties | Adherent |
| Recommended Medium | iXCells Fibroblast Growth Medium (MD-0011) |
| Expansion Capacity | Can further expand for 10 population doublings |
| Storage | Liquid nitrogen |
Handling
| Thawing | Place the vial in a 37 °C water bath for 1-2 minutes with gentle agitation, pipette into a tube with 5 mL medium, centrifuge at 1,000 rpm for 5 minutes |
|---|---|
| Subculture | At 80-90% confluence wash with DPBS, treat with 0.05% Trypsin-EDTA for 5 minutes at 37 °C, neutralise with 2-3 volumes of medium, centrifuge at 1,000 rpm for 5 minutes |
| Reseeding Density | 5,000-10,000 cells/cm2 |
Quality Control
- Express fibronectin and vimentin.
- Tested negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
Common Research Applications
- IPF disease modelling and anti-fibrotic compound screening.
- Myofibroblast differentiation and matrix-deposition assays.
- Extracellular matrix remodelling and contraction studies.
- Side-by-side comparison against normal-donor pulmonary fibroblasts.
Notes for Experimental Interpretation
- Pair with normal-donor pulmonary fibroblasts; the disease phenotype is only interpretable against a matched healthy control.
- Expansion is limited to about 10 population doublings - the fibrotic phenotype drifts with extended passaging.
- Record donor and lot with every experiment; IPF donor material is inherently variable.
Safety & Intended Use
Intended UseResearch Use Only (RUO)VendoriXCells BiotechnologiesCatalog No.10HU-240
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