| Field | Specification |
|---|---|
| Target | |
| Alternative names | cDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor ; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: Q24-A344 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human advanced glycation end product-specific receptor in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Kit Components
| Anti-Human AGER Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human AGER Standard | 2 vials, 10 ng/tube |
| Human AGER Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 78 pg/mL–5,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human Advanced glycation end product-specific receptor (Gln24–Ala344), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.4–7.2% (3 samples, n = 16 each) |
| Inter-assay CV | 5.5–7.5% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 294–5224 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.084; 78 = 0.156; 156 = 0.204; 312 = 0.319; 625 = 0.419; 1250 = 0.628; 2500 = 1.014; 5000 = 1.819. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.084 | 0.156 | 0.204 | 0.319 | 0.419 | 0.628 | 1.014 | 1.819 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 154 | 623 | 3076 | 159 | 595 | 3344 |
| Standard deviation | 10.16 | 27.41 | 221.47 | 10.65 | 32.72 | 250.8 |
| CV (%) | 6.6% | 4.4% | 7.2% | 6.7% | 5.5% | 7.5% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Yannan Chen et al. (2023) Effects of Tai Chi Chuan on Cognitive Function in Adults 60 Years or Older With Type 2 Diabetes and Mild Cognitive Impairment in China: A Randomized Clinical Trial. JAMA Network Open. 10.1001/jamanetworkopen.2023.7004
Elena Galluccio et al. (2021) Long lasting protective effects of early l-arginine treatment on endothelium in an in vitro study. Clinical Nutrition. 10.1016/j.clnu.2021.02.040
Kopytek Magdalena et al. (2020) Accumulation of advanced glycation end products (AGEs) is associated with the severity of aortic stenosis in patients with concomitant type 2 diabetes. Cardiovascular Diabetology. 10.1186/s12933-020-01068-7
Guangyan Liu et al. (2022) PM2.5 deregulated microRNA and inflammatory microenvironment in lung injury. Environmental Toxicology and Pharmacology. 10.1016/j.etap.2022.103832
Ying Zhang et al. (2025) Correlation between serum advanced glycation end-products and their receptor-mediated oxidative stress and perinatal outcomes in gestational diabetes mellitus. World Journal of Diabetes. 10.4239/wjd.v16.i6.104177
Chen Weiping et al. (2016) Accumulation of Advanced Glycation End Products Involved in Inflammation and Contributing to Severe Preeclampsia, in Maternal Blood, Umbilical Blood and Placental Tissues. Gynecologic and Obstetric Investigation. 10.1159/000448141
