| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinol-binding protein 4;Plasma retinol-binding protein;PRBP;RBP;Plasma retinol-binding protein (1-182);Plasma retinol-binding protein (1-181);Plasma retinol-binding protein (1-179);Plasma retinol-binding protein (1-176);RBP4;PRO2222; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: E19-L201 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma, Urine |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human retinol-binding protein 4 (gene RBP4) in cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA) and urine. Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Retinol-binding protein 4 (RBP4) is the main plasma carrier of vitamin A. Produced mostly by the liver, it transports retinol in blood, often in complex with transthyretin, and delivers it to tissues through the receptor STRA6. RBP4 is also released by fat tissue and is measured in studies of vitamin A status, insulin resistance and metabolic disease.
Kit Components
| Anti-Human RBP4 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human RBP4 Standard | 2 vials, 20 ng/tube |
| Human RBP4 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum, plasma(heparin, EDTA) and urine |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human RBP4 (Glu19–Leu201), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 7.1–7.9% (3 samples, n = 16 each) |
| Inter-assay CV | 7.4–8.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:20000. In the supplier’s tests, serum and plasma samples measured about 28 µg/mL.
Example standard curve (OD450, ng/mL): 0 = 0.044; 0.312 = 0.129; 0.62 = 0.218; 1.25 = 0.341; 2.5 = 0.569; 5 = 0.937; 10 = 1.445; 20 = 2.112. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.044 | 0.129 | 0.218 | 0.341 | 0.569 | 0.937 | 1.445 | 2.112 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 621 | 2874 | 9419 | 643 | 2778 | 10172 |
| Standard deviation | 44.09 | 227.04 | 668.74 | 53.36 | 247.24 | 752.72 |
| CV (%) | 7.1% | 7.9% | 7.1% | 8.3% | 8.9% | 7.4% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Melissa A McDiarmid et al. (2024) Thirty years of surveillance of depleted uranium-exposed Gulf War veterans demonstrate continued effects to bone health. Journal of Toxicology and Environmental Health-Part a-Current Issues. 10.1080/15287394.2024.2432021
Karadag Ayse Serap et al. (2013) The Effect of Acitretin Treatment on Insulin Resistance, Retinol-Binding Protein-4, Leptin, and Adiponectin in Psoriasis Vulgaris: A Noncontrolled Study. Dermatology. 10.1159/000351769
Karadag Ayse Serap et al. (2015) The Effect of Isotretinoin on Retinol-Binding Protein 4, Leptin, Adiponectin and Insulin Resistance in Acne Vulgaris Patients. Dermatology. 10.1159/000367687
Yılmaz Dilek et al. (2016) Evaluation of Nutritional Status in Children during Predialysis, or Treated By Peritoneal Dialysis or Hemodialysis. Journal of Tropical Pediatrics. 10.1093/tropej/fmv094
Payam FARAHBAKHSH-FARSI et al. (2016) Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients. Iranian Journal of Public Health. PMID: 27114982
