| Field | Specification |
|---|---|
| Target | |
| Alternative names | Resistin;Adipose tissue-specific secretory factor;ADSF;C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein;Cysteine-rich secreted protein A12-alpha-like 2;Cysteine-rich secreted protein FIZZ3;RETN;FIZZ3, HXCP1, RSTN;UNQ407/PRO1199; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: K19-P108 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human resistin (gene RETN) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Resistin (RETN) is a small cysteine-rich secreted protein. In mice it is made by adipocytes and was named for its ability to cause insulin resistance, whereas in humans it comes mainly from monocytes and macrophages and is tied more closely to inflammation. It is measured in studies of obesity, insulin resistance and inflammatory disease.
Kit Components
| Anti-Human RETN Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human RETN Standard | 2 vials, 10 ng/tube |
| Human RETN Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 78 pg/mL–5,000 pg/mL |
| Sensitivity | <3 pg/mL |
| Standard | Recombinant human RETN (Lys19–Pro108), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 6.0–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 6.0–7.9% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:10. In the supplier’s tests, serum and plasma samples measured about 20 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.02; 78 = 0.057; 156 = 0.093; 312 = 0.167; 625 = 0.311; 1250 = 0.629; 2500 = 1.164; 5000 = 1.979. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.02 | 0.057 | 0.093 | 0.167 | 0.311 | 0.629 | 1.164 | 1.979 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 135 | 639 | 2067 | 142 | 636 | 2227 |
| Standard deviation | 8.1 | 40.25 | 161.22 | 8.52 | 46.42 | 177.83 |
| CV (%) | 6% | 7.3% | 7.8% | 6% | 7.3% | 7.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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David R. Goldsmith et al. (2020) Protein and gene markers of metabolic dysfunction and inflammation together associate with functional connectivity in reward and motor circuits in depression. Brain Behavior and Immunity. 10.1016/j.bbi.2020.05.013
Mandakh Bekhbat et al. (2018) Glucose and lipid-related biomarkers and the antidepressant response to infliximab in patients with treatment-resistant depression. Psychoneuroendocrinology. 10.1016/j.psyneuen.2018.09.004
Smaranika Rahman et al. (2022) Increased serum resistin but not G-CSF levels are associated in the pathophysiology of major depressive disorder: Findings from a case-control study. PLoS One. 10.1371/journal.pone.0264404
Li Juan et al. (2019) Oxidative stress and adipokine levels were significantly correlated in diabetic patients with hyperglycemic crises. Diabetology & Metabolic Syndrome. 10.1186/s13098-019-0410-5
Yaşar Enli et al. (2014) Adipocytokine concentrations in children with different types of beta-thalassemia. Scandinavian Journal of Clinical & Laboratory Investigation. 10.3109/00365513.2014.883639
Ting-Yung Chang et al. (2020) Resistin as a Biomarker for the Prediction of Left Atrial Substrate and Recurrence in Patients with Drug-Refractory Atrial Fibrillation Undergoing Catheter Ablation. International Heart Journal. 10.1536/ihj.19-680
Xiao-hong Niu et al. (2017) Serum resistin positively correlates with serum lipids, but not with insulin resistance, in first-degree relatives of type-2 diabetes patients: an observational study in China. Medicine. 10.1097/MD.0000000000006622
Xinxin Zhu et al. (2023) Correlation of plasma adipokines with endometrial atypical hyperplasia and type I/II endometrial cancer. Journal of Obstetrics and Gynaecology. 10.1080/01443615.2023.2179914
Montazerifar Farzaneh et al. (2016) Obesity, Serum Resistin and Leptin Levels Linked to Coronary Artery Disease. Arquivos Brasileiros de Cardiologia. 10.5935/abc.20160134
Ali Buturak et al. (2016) Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study. Anatolian Journal of Cardiology. 10.14744/AnatolJCardiol.2016.6876
