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Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human SEMA3A concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA3A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA3A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SEMA3A. You can calculate the concentration of Human SEMA3A in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.1 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human SEMA3A in samples. No significant cross-reactivity or interference between Human SEMA3A and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.1 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant SEMA3A standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
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Osteoblasts are inherently programmed to repel sensory innervation (2020) Bone Research. 10.1038/s41413-020-0096-1
Axon guidance molecule semaphorin3A is a novel tumor suppressor in head and neck squamous cell carcinoma (2016) Oncotarget. 10.18632/oncotarget.6831
Parbendazole induces semaphorin 3A expression via JNK/c-Jun signaling pathway in normal human epidermal keratinocytes (2026) JOURNAL OF DERMATOLOGICAL SCIENCE. 10.1016/j.jdermsci.2025.12.001
Gingival crevicular fluid lipocalin-2 and semaphorin3A in stage III periodontitis: Non-surgical periodontal treatment effects (2022) JOURNAL OF PERIODONTAL RESEARCH. 10.1111/jre.12995
Assessment of semaphorin 3A and semaphorin 7A levels in primary Sjogren's syndrome (2022) RHEUMATOLOGY INTERNATIONAL. 10.1007/s00296-022-05197-y
Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes (2021) PLoS One. 10.1371/journal.pone.0250663
SEMA3A suspended in matrigel improves titanium implant fixation in ovariectomized rats (2016) JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS. 10.1002/jbm.b.33730
Identification and Functional Characterization of a Novel Variant in the SEMA3A Gene in a Chinese Family with Kallmann Syndrome (2022) International Journal of Endocrinology. 10.1155/2022/2504660
Decreased expression of Sema3A, an immune modulator, in blood sample of multiple sclerosis patients (2017) GENE. 10.1016/j.gene.2017.02.013
Semaphorin-3A as An Immune Modulator Is Suppressed by MicroRNA-145-5p (2018) Cell Journal. 10.22074/cellj.2018.4842
Evaluation of serum semaphorin 3A and interleukin 6 levels in patients with pseudoexfoliation syndrome (2022) INTERNATIONAL OPHTHALMOLOGY. 10.1007/s10792-022-02599-6
Plasma Semaphorin 3A as a Potential Biomarker for Cognitive Impairment in Cerebral Small Vessel Disease (2025) International Journal of General Medicine. 10.2147/IJGM.S554075
The Neuropilin-1 Ligand, Sema3A, Acts as a Tumor Suppressor in the Pathogenesis of Acute Leukemia (2018) Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology. 10.1002/ar.24016
Semaphorin 3A levels in vascular and nonvascular phenotypes in systemic sclerosis (2023) LABORATORY MEDICINE. 10.1093/labmed/lmad019
Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using MicroRNA-497-5p (2019) Iranian Journal of Medical Sciences. 10.30476/IJMS.2019.44955
