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Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human SIRT1 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.19 ng/mL |
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| Detection Range | 0.31-20 ng/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human SIRT1 in samples. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.19 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant SIRT1 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
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HMGB 1 acetylation mediates trichloroethylene-induced immune kidney injury by facilitating endothelial cell-podocyte communication (2023) ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY. 10.1016/j.ecoenv.2023.115042
The telomere-mitochondrial axis of aging in newborns (2022) Aging-US. 10.18632/aging.203897
Association between cognitive function and skeletal muscle in patients undergoing maintenance hemodialysis (2024) Frontiers in Endocrinology. 10.3389/fendo.2024.1324867
Serum sirtuin1: a potential blood biomarker for early diagnosis of Alzheimer’s disease (2023) Aging-US. 10.18632/aging.205015
In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells (2022) JOURNAL OF ETHNOPHARMACOLOGY. 10.1016/j.jep.2022.115110
SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease (2018) Oxidative Medicine and Cellular Longevity. 10.1155/2018/9391261
Phenolic Plant Extracts Induce Sirt1 Activity and Increase Antioxidant Levels in the Rabbit’s Heart and Liver (2018) Oxidative Medicine and Cellular Longevity. 10.1155/2018/2731289
Antiaging activity of polyphenol rich Calophyllum inophyllum L. fruit extract in Saccharomyces cerevisiae BY611 yeast cells (2021) Food Bioscience. 10.1016/j.fbio.2021.101208
Elucidation of anti-human melanoma and anti-aging mechanisms of compounds from green seaweed Caulerpa racemosa (2024) Scientific Reports. 10.1038/s41598-024-78464-6
Klotho and SIRT1 changes from pre-diabetes to diabetes and pre-hypertension to hypertension (2021) Diabetology & Metabolic Syndrome. 10.1186/s13098-021-00736-2
Serum Sirtuin-1, HMGB1-TLR4, NF-KB and IL-6 Levels in Alzheimer’s: The Relation Between Neuroinflammatory Pathway and Severity of Dementia (2022) Current Alzheimer Research. 10.2174/1567205020666221226140721
The role of ferroptosis and oxidative stress in cognitive deficits among chronic schizophrenia patients: a multicenter investigation (2025) Schizophrenia. 10.1038/s41537-025-00555-8
Behavior of Complement System Effectors in Chronic and Acute Coronary Artery Disease (2025) Journal of Clinical Medicine. 10.3390/jcm14113947
Changes in MMP-9, T-SOD and SIRT-1 levels after non-surgical periodontal treatment (2025) BMC Oral Health. 10.1186/s12903-025-05610-5
Plasma levels of sirtuin and adiponectin in patients with primary open-angle glaucoma, exfoliative glaucoma, and healthy controls (2021) EUROPEAN JOURNAL OF OPHTHALMOLOGY. 10.1177/11206721211065216
Serum Levels of Nitric Oxide, SIRT1, MMP-9 and Telomerase in Children With Cognitive Disengagement Syndrome (2025) INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE. 10.1002/jdn.70024
A cross-sectional study on the comparison of serum SIRT-1 and MMP-9 levels of patients with bronchiectasis and healthy controls (2025) Pakistan Journal of Medical Sciences. 10.12669/pjms.41.4.10877
