| Field | Specification |
|---|---|
| Target | |
| Alternative names | KIAA1029; |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Sensitivity | |
| Detection range | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Synaptopodin is an actin-associated protein that may play a role in actin-based cell shape and motility. The name synaptopodin derives from the protein's associations with postsynaptic densities and dendritic spines and with renal podocytes. Mouse and human SYNPO share 84% amino acid identity. SYNPO contains a high proline content (20%), making it unlikely that SYNPO forms a globular domain. It has several potential phosphorylation sites and 2 PPxY motifs, which interact with WW domain proteins. Northern blot analysis of human cerebral cortex detected a 4.4-kb transcript. Western blot analysis detected synaptopodin in rat brain beginning at embryonic day 15, with maximal expression in adult animals.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate SYNPO in samples. An antibody specific for SYNPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNPO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | 78.12-5000 pg/mL |
| Sensitivity | 32 pg/mL |
| Species Reactivity | Human (Homo sapiens) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE16102HU |
| UniProt # | Q8N3V7 |
| Alternative Names | KIAA1029; |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for SYNPO research.
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