| Field | Specification |
|---|---|
| Assay Time | |
| Assay Type | |
| Detection Instrument(s) | Flow Cytometer (Luminex-compatible or equivalent) |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, Plasma, Cell Culture Supernatant |
| Shipping | |
| Storage |
Overview
Super-X Plex™ Flow Cytometry Assay components to measure Human Tie-1 in human Serum, Plasma, Cell Culture Supernatant or Cell Lysate samples. Can be custom Multi-Plexed with other assays in the same species group.
Target Analyte
Tie-1
Ordering Note
Order a Sample Diluent Kit to match your sample type: Serum/Plasma, Cell Culture Supernatant (DKC100), or Cell Lysate (DKL100). Cell Lysate Prep Buffer (CLP200) and Sample Dilution Buffer (SDL200) also available. Order the matching Starter Kit for a complete reagent set.
Kit Contents
- Antibody-Conjugated Beads
- Detection Antibody – Biotin
- Lyophilized Antigen Standard (group-specific, pre-mixed)
Assay Performance
- Sample Volume: 15.0 µL per test
- Cross-Reactivity (within panel): Negligible
- Intra-Assay CV: <10%
- Inter-Assay CV: <20%
- Recovery: 70–130%
- Sensitivity / Detection Range: See product datasheet for analyte-specific values
Storage
Store Refrigerated (4–8°C, in dark).
⚠ Caution
Contains Sodium Azide (0.05%). Dispose of contents carefully; flush with water to avoid reaction with lead drain lines.
| Parameter | Value |
|---|---|
| Sample Volume | 15.0 µL per test |
| Intra-Assay CV | <10% |
| Inter-Assay CV | <20% |
| Recovery | 70–130% |
| Cross-Reactivity (within panel) | Negligible |
| Approximate Assay Time | ~3 Hours |
Performance data sourced from Antigenix America product datasheet. Actual values may vary by lot. Refer to certificate of analysis supplied with kit.
What sample types are compatible?
This Human Super-X Plex™ assay is compatible with serum, plasma, cell culture supernatant, and cell lysate samples. A species-matched Sample Diluent Kit (DKH100 (Human/Primate Serum/Plasma)) must be ordered separately.
What instrument is required?
A flow cytometer capable of detecting PE fluorescence is required. The assay is compatible with Luminex-based platforms and standard flow cytometers with bead-based multiplex detection capability.
Can this assay be combined into a custom multiplex panel?
Yes. Individual Super-X Plex™ assays within the same species group can be combined into custom multiplex configurations (up to 24-plex). Contact Antigenix for group compatibility details.
How much sample is needed per test?
15 µL per test. 96 tests kit provides sufficient reagents for a full standard curve plus samples.
Looking for a specific analyte, species, or panel configuration not listed? The Super-X Plex™ platform is designed for flexibility — from off-the-shelf single-plex assays to fully configured custom multi-plex panels and bespoke kit builds.
Custom Multi-Plex Panel Design
Individual Super-X Plex™ assays within the same species group can be combined into custom multiplex configurations without additional cross-reactivity validation. Select any combination of analytes from the available group catalog — from 2-plex to 24-plex — and receive a single pre-mixed standard and matched bead set. Custom panels are available for Human, Mouse, Rat, Canine, and Non-Human Primate matrices.
Custom Kit Services
Beyond panel configuration, BioHippo and Antigenix America offer extended custom kit services to support specialized study designs:
- Analyte selection: Access to the full Super-X Plex™ catalog, including analytes not listed online. If your target is not shown, contact us — additional cytokines, chemokines, growth factors, and soluble receptors may be available.
- Species expansion: Custom assay development for species beyond the standard catalog (Equine, Feline, Porcine, Bovine, and others) may be available upon inquiry.
- Sample matrix compatibility: Assays are validated for serum, plasma, cell culture supernatant, and cell lysate. Custom matrix validation or adjusted diluent formulations can be explored for non-standard sample types.
- Kit size and format: Standard kits are supplied as 96-test formats. Smaller pilot formats (32-test) are available for select panels. Bulk quantities and custom packaging for high-throughput or multi-site studies can be quoted on request.
- Pre-mixed standard panels: Custom pre-mixed lyophilized standards can be formulated to match your exact panel composition, simplifying standard curve preparation across multiple assay runs.
- White-label and OEM supply: For core facilities, CROs, or institutional programs requiring custom-labeled or privately packaged kits, OEM supply arrangements can be discussed.
How to Request
Click Talk to a Scientist to submit a customization request form, email us at support@biohippo.com, or explore our Research Services for additional support options. Our team will review your requirements and respond promptly.
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