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Scientific Background
This ELISA kit applies to the in vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids.
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-2. You can calculate the concentration of Human TIMP-2 in the samples by comparing the OD of the samples to the standard curve.
Performance Specifications
| Sensitivity | 0.1 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
| Total Assay Time | 3 h 30 min |
| Compatible Sample Types | Serum, plasma and other biological fluids |
| Species Reactivity | Human |
| Detection Method | Sandwich |
| Precision (CV) | Both intra-CV and inter-CV are < 10%. |
| Recovery Rate | 80%-120% |
| Storage | 2-8℃,12 months |
✓ Research-Grade Validation
Specificity
This kit recognizes Human TIMP-2 in samples. No significant cross-reactivity or interference between Human TIMP-2 and analogues was observed
Safety & Regulatory
Handle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.
This kit is validated for use with Serum, plasma and other biological fluids. For unlisted matrices (e.g., tissue lysate, urine), perform a spike-and-recovery experiment to confirm assay performance before generating reportable data. Sample dilution in the kit's provided diluent is recommended to minimize matrix interference.
The minimum detectable concentration (sensitivity) of this kit is 0.1 ng/mL. Values below this threshold should be reported as below the limit of detection (<LOD) and should not be extrapolated from the standard curve.
The total assay time from sample addition to absorbance reading is approximately 3 h 30 min, including all incubation, wash, and substrate steps. Hands-on time is typically 1–2 hours; most steps involve passive plate incubation. Plan the assay as a single uninterrupted session for best results.
Standard components of this Sandwich ELISA Kit typically include: pre-coated microplate (96-well strip format), lyophilized or liquid recombinant TIMP-2 standard, detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample/standard diluent. Refer to the kit insert or datasheet for the exact component list and storage requirements.
This kit uses colorimetric (TMB/HRP) detection and requires a standard microplate absorbance reader capable of measuring at 450 nm. A reference wavelength of 570 nm or 630 nm is recommended to reduce background. No specialized fluorescence or luminescence reader is needed. Ensure the instrument is calibrated and the plate is clean and free of condensation before reading.
Tissue samples may contain endogenous or exogenous proteases during processing, leading to degradation of extracted proteins. Therefore, it's necessary to add protease inhibitors during processing to ensure the integrity of target proteins. If customers can keep samples cold and handle them quickly during processing, omitting the protease inhibitor may not have a significant effect. After preparation, samples should be tested promptly or immediately aliquoted and frozen at -20°C or -80°C.
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Single urinary extracellular vesicle proteomics identifies complement receptor CD35 as a biomarker for sepsis-associated acute kidney injury (2025) Nature Communications. 10.1038/s41467-025-62229-4
Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis (2023) BIOMEDICINE & PHARMACOTHERAPY. 10.1016/j.biopha.2023.114289
Three kinds of corneal host cells contribute differently to corneal neovascularization (2019) EBioMedicine. 10.1016/j.ebiom.2019.05.026
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases (2021) Cells. 10.3390/cells10123506
TIMP-2 regulates 5-Fu resistance via the ERK/MAPK signaling pathway in colorectal cancer (2022) Aging-US. 10.18632/aging.203793
Interference of Immunosuppressive Therapies with Cellular Antimicrobial Activity Against Mycobacterium abscessus (2026) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 10.3390/ijms27073230
Kisspeptin-10 increases collagen content in the myocardium by focal adhesion kinase activity (2023) Scientific Reports. 10.1038/s41598-023-47224-3
TIMP-2 Modulates 5-Fu Resistance in Colorectal Cancer Through Regulating JAK–STAT Signalling Pathway (2025) JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 10.1111/jcmm.70470
Preclinical efficacy and safety evaluation of a topical mesenchymal stem cell derived conditioned media gel for oral mucositis management (2026) Scientific Reports. 10.1038/s41598-026-40193-3
TIMP-2 as a predictive biomarker in 5-Fu-resistant colorectal cancer (2023) JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY. 10.1007/s00432-023-04670-w
Urinary NGAL, IGFBP-7, and TIMP-2: novel biomarkers to predict contrast medium-induced acute kidney injury in children (2022) RENAL FAILURE. 10.1080/0886022X.2022.2075277
The Predictive Role of MMP-2, MMP-9, TIMP-1 and TIMP-2 Serum Levels in the Complete Response of the Tumor to Chemotherapy in Breast Cancer Patients (2022) JOURNAL OF INVESTIGATIVE SURGERY. 10.1080/08941939.2022.2080308
MT1-MMP evaluation in neointimal hyperplasia in the late follow-up after prosthesis implantation (2019) INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY. 10.1111/iep.12310
The Potential Role of Serum Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) as a Biomarker of Fibrosis in Patients With Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) (2025) Cureus Journal of Medical Science. 10.7759/cureus.96047
