| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | serum, tissue |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Assay Principle
The Tribo TM Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours instead of 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL . The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.
Key Features Novel Proprietary ELISA Approach
Simple: Sample and Detection combined into one step.
Human TNF-α ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of TNF-α. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | TNF-α |
|---|---|
| Species | Human |
| Sample Type | serum, tissue |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 10 pg/mL |
| Detection Range | 8–2000 pg/mL |
| Assay Time | 3 hours |
| Precision | Intra-assay CV 3.79%; Inter-assay CV 10% |
| Standard | Recombinant Human TNF-α |
| Specificity | This assay recognizes natural and recombinant human TNF-α. |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for serum, tissue. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 10 pg/mL and a detection range of 8–2000 pg/mL. Samples reading outside the range should be diluted or concentrated.
The total assay time is approximately 3 hours from sample addition to plate reading.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
