| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2 |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sensitivity | |
| Target | |
| UniProt # |
Scientific background
TNFa (Tumor Necrosis Factor Alpha) is a TNF-family inflammatory mediator involved in cytokine cascades and cell fate signaling (survival/apoptosis), depending on context.
TNF-pathway markers are commonly monitored in innate immune activation and inflammatory disease models and can respond to endotoxin or immune-modulating treatments.
Protein-level quantification helps compare cohorts and treatment effects, especially when transcriptional changes do not directly predict secreted protein abundance.
Why it matters
- Quantify TNFa (Tumor Necrosis Factor Alpha) to compare biological changes across conditions, doses, or time points.
- Generate concentration data from a standard curve to support biomarker and mechanistic studies.
How the ELISA works
Designed for Human samples, this kit uses a The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.. After binding and washing, signal is converted to concentration using a standard curve.
Sample types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Detection range: 15.63-1000 pg/mL
- Sensitivity/LoD: 6.4 pg/mL
- Assay time: 3.5h
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