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In the DNA decatenation reaction, human DNA topoisomerase II turns concatenated DNA into decatenated DNA.
Formats and Pack Sizes
HDC020K has no enzyme. HDC020KE has the same 20-assay reagents plus human topoisomerase II alpha, and HDC100KE is the enzyme-included kit for 100 assays.
| Kit (enzyme not included), 20 assays (HDC020K) | 20 assays |
|---|---|
| Kit Plus (enzyme included), 20 assays (HDC020KE) | 20 assays |
| Kit Plus (enzyme included), 100 assays (HDC100KE) | 100 assays |
Kit Components
| Component | Kit (enzyme not included), 20 assays (HDC020K) | Kit Plus (enzyme included), 20 assays (HDC020KE) | Kit Plus (enzyme included), 100 assays (HDC100KE) |
|---|---|---|---|
| Assay buffer | Included | Included | Included |
| Concatenated DNA | Included | Included | Included |
| Spin columns | Included | Included | Included |
| Loading solution | Included | Included | Included |
| Human topoisomerase II alpha | Not included | Included | Included |
Assay Principle
Human topoisomerase II alpha decatenates (unlinks) the supplied concatenated DNA. The kit supplies spin columns and a loading solution for processing the reaction products.
Specifications
| Assay Target | Human topo II DNA decatenation |
|---|---|
| Target Type | Topoisomerase |
| Organism | Human |
| Assay Type | Enzyme Activity Assay; Inhibitor Screening Assay |
| Sizes | 20 assays, 100 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
The manufacturer's protocol describes the procedure and reaction volumes.
- Spin-Column Human Topoisomerase II Assay (PDF, see Documents): Covers catalog # HDC020K, HDC020KE, HDC100KE.
Safety & Handling
Can’t find the exact format you need? We can help you source or customize this human topo II DNA decatenation assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
