| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Product Type | |
| Sample Type(s) | cell lysate |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
GAPDH (Glyceraldehyde-3-phosphate Dehydrogenase) is an enzyme that is involved in the second phase of glycolysis, a process that breaks down glucose and ultimately produces pyruvate for the trichloroacetic acid cycle (TCA cycle), and adenosine triphosphate (ATP), which is the primary energy source for important biological functions.
Assay Principle
GAPDH is generally thought to be present at a constant level within the cell. In most cases, this stays true regardless of experimental treatment or technical procedures. Because of its ability to remain present, the measurement of GAPDH is used as an internal control for experimental error. GAPDH is widely used in different assay methods to investigate certain aspects of cell activity.
The Human Total GAPDH ELISA Kit is used to measure the presence of human GAPDH in cell lysates.
Typical Data This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.
Human Total GAPDH ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of Total GAPDH. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Total GAPDH |
|---|---|
| Species | Human |
| Sample Type | cell lysate |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 0.15625 ng/mL |
| Standard | Recombinant Human Total GAPDH |
| Storage | Store the unopened kit at 2-8°C. | Components: The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell lysate. Other matrices may require additional in-lab validation.
This assay has a sensitivity of 0.15625 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
