| Field | Specification |
|---|---|
| Applications | |
| Assay type | |
| Sample type(s) | Serum | Plasma |
| Sensitivity | |
| Detection range | |
| Detection method | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The Human Toxoplasma IgG ELISA Kit quantifies human IgG antibodies against Toxoplasma in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.
Values are expressed relative to the antibody standard supplied with the kit.
Specifications
| Assay Type | Quantitative |
|---|---|
| Detection Method | Colorimetric (OD 450 nm) |
| Sample Type | Serum, Plasma |
| Sensitivity | 0.25 ng/mL |
| Detection Range | 1.56 - 100 ng/mL |
| Recovery | 80-120% |
| Kit Size | 96 T |
| Shipping | 2-8 °C |
Kit Components
| Component | Quantity |
|---|---|
| ELISA Microtiter plate | 96 wells |
| Positive Control | 1 mL |
| Negative Control | 1 mL |
| HRP Conjugate | 12 mL |
| Sample Diluent | 12 mL |
| 20×Concentrated Wash Buffer | 50 mL |
| Substrate Reagent A | 6 mL |
| Substrate Reagent B | 6 mL |
| Stop Solution | 6 mL |
| Plate Sealer | 3 pieces |
| Sealed Bag | 1 piece |
| Manual | 1 copy |
Assay Procedure
Bring all reagents to room temperature for 30 min. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.
- Number the sample and control in order (in duplicate), and keep a record of control wells and sample wells. Set 1 well for blank control (empty), 3 wells for negative control and 1 well for positive control. Run samples in duplicate.
- Add 100 µL positive/negative control to positive/negative control well. (A blank well is not needed with dual-wavelength reading). Dilute the tested serum with Sample Diluent at 1:10 (add 100 µL of Sample Diluent to the reaction well, and then add 10 µL of serum sample), mix fully.
- Cover with a plate sealer and mix thoroughly, incubate at 37 °C for 30 min.
- After incubation, remove the plate sealer and aspirate the liquid of each well. Repeat the washing procedure for 5 times with Wash Buffer and immerse for 30-60 sec each time.
- Add 100 µL of HRP Conjugate to each well except the blank control well.
- Cover the ELISA plate with sealer. Incubate for 30 min at 37 °C.
- Repeat step 4 for washing.
- Add 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B to each well. Gently tap the plate to mix thoroughly. Cover with a new plate sealer. Incubate for 10 min at 37 °C protected from light.
- Add 50 µL of Stop Solution to each well, gently tap the plate to mix thoroughly.
- Set the Micro-plate Reader wavelength at 450 nm (it is recommended to set the dual wavelength at 450 nm/630 nm) to detect A value of each well. Blank well is not essential when using dual wavelength 450 nm/630 nm for detection. Note: Read the results within 30 min.
Required but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm / 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.
Storage & Handling
For Research Use Only. Not for use in diagnostic procedures.
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