| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transthyretin; ATTR; Prealbumin; TBPA; TTR; PALB |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: G21-E147 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human transthyretin (gene TTR) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Transthyretin (TTR) is a tetrameric transport protein made mainly by the liver and the choroid plexus. It carries thyroxine in blood and cerebrospinal fluid and transports retinol by binding retinol-binding protein. Destabilized TTR can misfold into amyloid fibrils, causing transthyretin amyloidosis.
Kit Components
| Anti-Human TTR Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human TTR Standard | 2 vials, 20 ng/tube |
| Human TTR Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (heparin, EDTA) |
| Assay range | 312 pg/mL–20,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human TTR (Gly21–Glu147), expressed in E. coli |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 3.4–7.5% (3 samples, n = 16 each) |
| Inter-assay CV | 2.0–6.3% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1000000. In the supplier’s tests, serum and plasma samples measured about 240 µg/mL.
Example standard curve (OD450, ng/mL): 0 = 0.036; 0.312 = 0.11; 0.62 = 0.155; 1.25 = 0.254; 2.5 = 0.479; 5 = 0.841; 10 = 1.418; 20 = 2.047. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.036 | 0.11 | 0.155 | 0.254 | 0.479 | 0.841 | 1.418 | 2.047 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 456 | 1624 | 9289 | 529 | 1947 | 9298 |
| Standard deviation | 34.34 | 55.87 | 599.02 | 33.49 | 38.94 | 304.04 |
| CV (%) | 7.5% | 3.4% | 6.4% | 6.3% | 2% | 3.3% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Jiyong Yin et al. (2023) Construction and Evaluation of a Novel MAP Immunoassay for 9 Nutrition-and-Health-Related Protein Markers Based on Multiplex Liquid Protein Chip Technique. Nutrients. 10.3390/nu15061522
Mathuramat Seesen et al. (2021) Cognitive Frailty in Thai Community-Dwelling Elderly: Prevalence and Its Association with Malnutrition. Nutrients. 10.3390/nu13124239
Yasin Öztürk et al. (2026) Multiple contaminant detection in commercially available chicken-based cat kibbles in Italy. Frontiers in Veterinary Science. 10.3389/fvets.2026.1846767
Wen Jinkun et al. (2021) Methylation silencing and reactivation of exogenous genes in lentivirus-mediated transgenic mice. Transgenic Research. 10.1007/s11248-020-00224-9
Liangyu Fei et al. (2025) Effect of hybrid blood purification on nutritional status, inflammation, and cardiovascular events in patients with end-stage renal disease. Pakistan Journal of Medical Sciences. 10.12669/pjms.41.1.10556
Hongmin Chen et al. (2025) Comparative effects of enteral nutrition strategies on nutritional indices, inflammatory factors, and clinical outcomes in patients with severe traumatic brain injury. American Journal of Translational Research. 10.62347/QROO6899
