Human Umbilical Mesenchymal Stem Cells (HUMSC)

Overview

Human Umbilical Mesenchymal Stem Cells (HUMSC) is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Mesenchymal Stem Cells derived from Umbilical Cord (Umbilical) within the Reproductive system.

Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine. MSC isolated from the Wharton’s jelly of the umbilical cord were induced to transform into neurons and glia in vitro by using neuron-conditioned medium, sonic hedgehog, and FGF-8 [1, 2] . MSC can also differentiate into cells from the adipogenic and osteogenic lineage. Additionally, they have the potential to differentiate into cardiomyocytes by culturing them in cardiomyocyte-conditioned medium [3] . MSC express the matrix receptors CD44 and CD105 and mesenchymal stem cell markers SH2 and SH3, but not the hematopoietic lineage marker CD34. Human Umbilical Mesenchymal Stem Cells (HUMSC)from iXCells Biotechnologies are isolated from Wharton’s jelly of the umbilical cord. HUMSC are cryopreserved at passage one and delivered frozen. Each vial contains 0.5 million cells in 1 ml volume. HUMSC are characterized by immunofluorescence with antibodies specific to CD73, CD90 and CD105. HUMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUMSC can expand for no more than 3 passages under the conditions recommended by iXCells Biotechnologies using Mesenchymal Stem Cell Medium (Cat# MD-0037) . Figure 1. Human Umbilical Mesenchymal Stem Cells (HUMSC). ( A ) Phase contrast image of HUMSC. ( B ) Immunofluorescence staining with antibodies against CD29 and CD90.

Key elements and design rationale

• Cell identity: Mesenchymal Stem Cells (Primary Cells)
• Source context: Umbilical Cord; Umbilical; Reproductive
• Donor background: Age: Adult
• Biosafety level: BSL-2 (follow your institution’s biosafety program and local regulations)

Product-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.

Biological background

Stem and progenitor cell models are widely used to study differentiation programs, lineage commitment, and regenerative responses under controlled culture perturbations.

Across primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.

Research relevance and current trends

• Increasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.
• Adoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.
• Integration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.

Common research applications

• Profile identity markers by flow cytometry or immunostaining in cultured cells
• Induce lineage differentiation and track marker changes over a maturation time-course
• Quantify functional responses to defined stimuli relevant to the model system
• Compare baseline phenotype across donors/conditions using gene expression profiling
• Evaluate multipotency using lineage-specific staining and gene expression panels

Interpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.

Notes for experimental interpretation

• Donor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.
• Passage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.
• Contamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.
• Use appropriate negative/positive controls for the readout (e.g., unstimulated controls, pathway agonists/antagonists) to contextualize observed changes.

SKU:BHC18500059

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