| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;INCAM-100;CD106;VCAM1;L1CAM; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: F25-E698 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human vascular cell adhesion protein 1 (gene VCAM1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
VCAM-1 (CD106) is an immunoglobulin-superfamily adhesion molecule expressed on activated endothelial cells. Induced by inflammatory cytokines, it binds the integrin VLA-4 (α4β1) on leukocytes and supports their firm adhesion and migration into tissues. VCAM-1 is a common marker of endothelial activation in inflammation and atherosclerosis research.
Kit Components
| Anti-Human VCAM1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human VCAM1 Standard | 2 vials, 10 ng/tube |
| Human VCAM1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <10 pg/mL |
| Standard | Recombinant human VCAM1 (Phe25–Glu698), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.8–7.3% (3 samples, n = 16 each) |
| Inter-assay CV | 6.4–8.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:300. In the supplier’s tests, serum and plasma samples measured about 500 ng/mL.
Example standard curve (OD450, pg/mL): 0 = 0.008; 156 = 0.068; 312 = 0.118; 625 = 0.236; 1250 = 0.463; 2500 = 0.797; 5000 = 1.32; 10000 = 2.023. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.008 | 0.068 | 0.118 | 0.236 | 0.463 | 0.797 | 1.32 | 2.023 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 310 | 1065 | 5287 | 304 | 1029 | 5603 |
| Standard deviation | 17.67 | 51.12 | 387.35 | 19.15 | 65.85 | 481.85 |
| CV (%) | 5.7% | 4.8% | 7.3% | 6.4% | 6.4% | 8.6% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Qing-Bo Lu et al. (2019) Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway. Redox Biology. 10.1016/j.redox.2019.101411
Zhuan Zhou et al. (2020) VCAM-1 secreted from cancer-associated fibroblasts enhances the growth and invasion of lung cancer cells through AKT and MAPK signaling. Cancer Letters. 10.1016/j.canlet.2019.12.039
Carla Gouin et al. (2023) Cell type- and time-dependent biological responses in ex vivo perfused lung grafts. Frontiers in Immunology. 10.3389/fimmu.2023.1142228
Xue-Xue Zhu et al. (2025) Sodium pump subunit NKAα1 protects against diabetic endothelial dysfunction by inhibiting ferroptosis through the autophagy-lysosome degradation of ACSL4. Clinical and Translational Medicine. 10.1002/ctm2.70221
Aya A. Eltaibany et al. (2026) Modeling Neuroimmunological Interactions at the Blood–Brain Barrier Using In Vitro 3D Human Organoids: Inflammation and Ischemia–Reperfusion Injury. Cells. 10.3390/cells15131173
Su Yingxue et al. (2019) MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis. Cell Death & Disease. 10.1038/s41419-019-1599-9
Mingwei Xu et al. (2022) Genetic Arg-304-His substitution in GRK5 protects against sepsis progression by alleviating NF-κB-mediated inflammation. International Immunopharmacology. 10.1016/j.intimp.2022.109629
Li Chenglong et al. (2022) sVCAM1 in the Hippocampus Contributes to Postoperative Cognitive Dysfunction in Mice by Inducing Microglial Activation Through the VLA-4 Receptor. Molecular Neurobiology. 10.1007/s12035-022-02924-1
K. Hashimoto et al. (2016) Soluble Adhesion Molecules During Ex Vivo Lung Perfusion Are Associated With Posttransplant Primary Graft Dysfunction. American Journal of Transplantation. 10.1111/ajt.14160
Xiaowei Zhang et al. (2013) Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection. Journal of Virology. 10.1128/JVI.02090-13
