| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor C;VEGF-C;Flt4 ligand;Flt4-L;Vascular endothelial growth factor-related protein;VRP;VEGFC; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: T103-R227 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Cell Lysate, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human vascular endothelial growth factor C (gene VEGFC) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
VEGF-C is a growth factor that acts mainly on lymphatic vessels. After proteolytic processing it binds VEGFR3 and, in its mature form, VEGFR2, driving lymphatic endothelial growth and lymphangiogenesis. VEGF-C is studied in lymphatic development, lymphedema and tumor lymphatic spread.
Kit Components
| Anti-Human VEGFC Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human VEGFC Standard | 2 vials, 10 ng/tube |
| Human VEGFC Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay range | 62.5 pg/mL–4,000 pg/mL |
| Sensitivity | <3 pg/mL |
| Standard | Recombinant human VEGFC (Thr103–Arg227), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.7–6.7% (3 samples, n = 16 each) |
| Inter-assay CV | 5.7–8.6% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Sample dilution: start at 1:1. In the supplier’s tests, serum and plasma samples measured 500–1250 pg/mL.
Example standard curve (OD450, pg/mL): 0 = 0.029; 62.5 = 0.063; 125 = 0.099; 250 = 0.181; 500 = 0.323; 1000 = 0.643; 2000 = 1.307; 4000 = 2.025. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.029 | 0.063 | 0.099 | 0.181 | 0.323 | 0.643 | 1.307 | 2.025 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 86 | 845 | 1351 | 89 | 836 | 1341 |
| Standard deviation | 4.04 | 56.61 | 72.95 | 5.51 | 71.89 | 76.43 |
| CV (%) | 4.7% | 6.7% | 5.4% | 6.2% | 8.6% | 5.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Dai Lei et al. (2016) SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin. Nature Communications. 10.1038/ncomms11996
Jie Bai et al. (2022) Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms. Oxidative Medicine and Cellular Longevity. 10.1155/2022/5044046
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Xindong Zhang et al. (2011) CYR61 Modulates the Vascular Endothelial Growth Factor C Expression of Decidual NK Cells Via PI3K/AKT Pathway. American Journal of Reproductive Immunology. 10.1111/j.1600-0897.2011.01080.x
Yun-Fei Xu et al. (2015) High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma. World Journal of Gastroenterology. 10.3748/wjg.v21.i11.3256
Sun Ping et al. (2007) RNA interference (RNAi)-mediated vascular endothelial growth factor-C (VEGF-C) reduction interferes with lymphangiogenesis and enhances Epirubicin sensitivity of breast cancer cells. Molecular and Cellular Biochemistry. 10.1007/s11010-007-9624-1
