| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vascular endothelial growth factor receptor 1;VEGFR-1;2.7.10.1;Fms-like tyrosine kinase 1;FLT-1;Tyrosine-protein kinase FRT;Tyrosine-protein kinase receptor FLT;FLT;Vascular permeability factor receptor;FLT1;FLT, FRT, VEGFR1; |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: NS0; Immunogen sequence: S27-H687 |
| Expression system | |
| Expression region | |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies human vascular endothelial growth factor receptor 1 (gene FLT1) in cell culture supernatants, serum and plasma (EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Vascular endothelial growth factor receptor 1 (VEGFR-1, FLT1) is a receptor tyrosine kinase that binds VEGF-A, VEGF-B and placental growth factor. Its kinase signaling is weak, and a soluble form acts largely as a decoy that limits VEGF availability. FLT1 is studied in angiogenesis, macrophage recruitment, preeclampsia and vascular development.
Kit Components
| Anti-Human FLT1 Pre-coated 96-well strip microplate | 1 plate (96 wells) |
|---|---|
| Human FLT1 Standard | 2 vials, 10 ng/tube |
| Human FLT1 Biotinylated antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Adhesive plate sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Kit components are not sold separately.
Performance Data
| Species | Human |
|---|---|
| Sample types | Cell culture supernatants, serum and plasma (EDTA) |
| Assay range | 156 pg/mL–10,000 pg/mL |
| Sensitivity | <30 pg/mL |
| Standard | Recombinant human FLT1 (Ser27–His687), expressed in NS0 |
| Cross-reactivity | None detected against related proteins in supplier testing |
| Intra-assay CV | 4.1–7.8% (3 samples, n = 16 each) |
| Inter-assay CV | 4.7–9.7% (3 samples, n = 24 each) |
| Format | 96 wells, removable strips |
Example standard curve (OD450, pg/mL): 0 = 0.012; 156 = 0.076; 312 = 0.126; 625 = 0.235; 1250 = 0.469; 2500 = 0.973; 5000 = 1.864; 10000 = 2.669. Run a new standard curve on every plate.
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.012 | 0.076 | 0.126 | 0.235 | 0.469 | 0.973 | 1.864 | 2.669 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 237 | 1369 | 4420 | 240 | 1352 | 4316 |
| Standard deviation | 9.71 | 106.78 | 203.32 | 11.28 | 131.14 | 202.85 |
| CV (%) | 4.1% | 7.8% | 4.6% | 4.7% | 9.7% | 4.7% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Materials required but not provided
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
Yıldız Hayran et al. (2019) Vascular endothelial growth factor gene polymorphisms in patients with rosacea: A case-control study. Journal of the American Academy of Dermatology. 10.1016/j.jaad.2019.03.055
Ruirui Hou et al. (2025) SIRT2 alleviates pre-eclampsia via prompting mitochondrial biogenesis and function. Life Sciences. 10.1016/j.lfs.2025.123566
Zhi-Hui Xiong et al. (2018) Protective effect of human umbilical cord mesenchymal stem cell exosomes on preserving the morphology and angiogenesis of placenta in rats with preeclampsia. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2018.06.032
Fengyuan Zhang et al. (2022) Distinguishing placenta accreta from placenta previa via maternal plasma levels of sFlt-1 and PLGF and the sFlt-1/PLGF ratio. Placenta. 10.1016/j.placenta.2022.05.009
Zhang Fangchao et al. (2024) Relationship between maternal serum sFlt-1 level and placenta accreta spectrum disorders in the third trimester. Archives of Gynecology and Obstetrics. 10.1007/s00404-024-07734-5
Eray Eroglu et al. (2020) Soluble vascular endothelial growth factor receptor-1 as a novel marker of arteriovenous fistula stenosis in hemodialysis patients. Hemodialysis International. 10.1111/hdi.12903
Tuba Bozduman et al. (2016) Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene. Annals of Dermatology. 10.5021/ad.2016.28.1.30
Kathleen R. Chirco et al. (2017) Evaluation of sFLT1 protein levels in human eyes with the FLT1 rs9943922 polymorphism. Ophthalmic Genetics. 10.1080/13816810.2017.1369550
Chase Cawyer et al. (2016) Attenuation of hyperglycemia-induced apoptotic signaling and anti-angiogenic milieu in cultured cytotrophoblast cells. Hypertension in Pregnancy. 10.3109/10641955.2015.1122035
Junru LIU et al. (2007) Soluble Fms-like Tyrosine Kinase-1 Expression Inhibits the Growth of Multiple Myeloma in Nude Mice. ACTA Biochimica et Biophysica Sinica. 10.1111/j.1745-7270.2007.00310.x
