| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
HVEM (herpesvirus entry mediator, TNFRSF14) is a tumor necrosis factor receptor superfamily member with a complex role in immune regulation. It serves as a molecular switch that can deliver either stimulatory or inhibitory signals depending on its binding partner: engagement of TNF-family ligands such as LIGHT promotes NF-κB-driven activation, whereas interaction with the immunoglobulin-family receptors BTLA and CD160 transmits inhibitory signals that suppress lymphocyte activation. Through this bidirectional signaling, HVEM helps balance immune activation and tolerance. Its function as a checkpoint and co-stimulatory hub makes HVEM a target of interest in immunotherapy, and NF-κB reporters provide a quantitative readout of HVEM pathway engagement.
Product Description & Applications
The HVEM/NF-κB Reporter Lentivirus is an immunotherapy reporter system for studying HVEM as an immune checkpoint molecule. The construct constitutively expresses the human HVEM receptor; engagement of inhibitory ligands such as BTLA suppresses NF-κB signaling and reduces reporter activity, while agents that block this inhibition restore NF-κB-driven reporter expression. Dual reporters, secreted Gaussia luciferase and a fluorescent protein (GFP or RFP), allow real-time monitoring by luminometry and fluorescence.
Applications include testing immune checkpoint inhibitors that target HVEM signaling and studying lymphocyte regulation. Supplied as high-titer, VSV-G-pseudotyped lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, the product is optimized for difficult-to-transfect primary and thawed cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human HVEM under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
