ID8 cell

SKU:BHC11101573
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Overview
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ID8 cell is a Epithelial cell cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Epithelial-like. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Mouse
Disease model Normal
Morphology Epithelial-like
Growth Properties Adherent
Tissue Ovary
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Catalog no. Size
305305 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305305
Species Mouse
The ID8 cell line is a widely utilized murine model derived from spontaneous transformation of C57BL/6 mouse ovarian surface epithelial (MOSE) cells. This cell line closely mimics human epithelial ovarian cancer, making it a vital tool for preclinical research into ovarian cancer pathophysiology and treatment. ID8 cells are known for their ability to grow intraperitoneally in immunocompetent C57BL/6 mice, facilitating studies of tumor progression and metastasis. This model is particularly relevant for examining peritoneal tumor formation and ascites development, which are key features of advanced ovarian cancer in patients. ID8 cells exhibit the capability to form tumors when injected intraperitoneally, leading to disseminated cancer throughout the abdominal cavity and ascitic fluid accumulation. These properties enable the exploration of tumor-host interactions, including the role of the immune system and tumor microenvironment in cancer progression. In studies involving immunotherapies or combined treatment approaches, ID8 has proven valuable for evaluating the effects of interventions such as chemotherapy agents like carboplatin and immune checkpoint inhibitors targeting PD-L1. Research involving ID8 models has shown their utility in examining the influence of tumor metabolism on immune cell behavior, particularly macrophage polarization and function. For instance, tumors induced by ID8 cells can modulate the metabolism of peritoneal macrophages, altering their oxidative phosphorylation (OXPHOS) and promoting tumor growth through metabolic crosstalk. These insights have paved the way for exploring targeted metabolic therapies that may inhibit tumor-promoting immune cell adaptations.

SKU:BHC11101573

  • cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
  • supplements: Supplement the medium with 10% FBS
  • dissociationReagent: Accutase
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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