| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
IdeS Protease, full name Immunoglobulin G-degrading enzyme of Streptococcus pyogenes, IdeS, is a cysteine protease produced and secreted extracellularly by the human pathogen Streptococcus pyogenes. This proteinase has extremely high substrate specificity, only recognizing IgG and cleaving at a specific site in the antibody's hinge region, resulting in the hydrolysis of IgG into intact F(ab’)2 fragments and Fc fragments. IdeS can recognize human and various other animal sources of IgG, such as human, rabbit, monkey, sheep, and human-animal chimeric IgG, etc. IdeS has unique substrate selectivity and can be used as a tool enzyme for structural characterization analysis of antibody drugs or antibody-fusion protein drugs.
This product is recombinantly expressed in E. coli, with high purity and good enzymatic activity, making it a valuable tool for characterizing therapeutic antibodies, Fc fusion proteins, and antibody-drug conjugates.
Features
High Specificity — Single cleavage site: CPAPELLG/GPSVF.
Fast Reaction — 30 minutes rapid cleavage, significantly faster than papaya proteinase and pepsin.
High Stability — Each batch of product undergoes strict quality control to ensure batch-to-batch stability.
Simple Reaction Conditions — Compatible with various pH buffer solutions, no need for reducing agents or auxiliary reagents.
Applications
IdeS enzyme is used as a tool enzyme in the preparation and structural characterization analysis of antibody drugs.
IdeS enzyme can also assist in drug development in certain fields.
Specifications
|
English synonym |
IdeS Protease |
|
Source |
E.coli recombinant expression |
|
Label |
N-terminal His Tag |
|
Purity |
Purity is greater than 95% as analyzed by SDS-PAGE and HPLC |
|
Molecular Weight |
35.3 KDa |
|
Storage buffer |
50 mM sodium phosphate, 150 mM NaCl (pH 6.6), 50% glycerol |
|
Enzyme Concentration |
40 U/μL |
|
Unit Definition |
The amount of enzyme required to cleave more than 95% of 1 μg of recombinant monoclonal IgG in 30 minutes at 37°C is defined as one unit of activity. |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
20412ES84 |
20412ES90 |
|
20412 |
IdeS Protease |
2000 U |
5000 U |
Shipping and Storage
The products can be stored at -15℃ ~ -25℃ for one year.
Figure
Figure 1. A. The HPLC result of MAG3-Cet-F(ab′)2 and MAG3-Cet-Fc after digesting MAG3-Cet with IdeS protease. The HPLC result of the mixture from (A) but after reaction with protein A beads. The residual MAG3-Cet and most of the MAG3-Cet-Fc were removed by protein A beads.
Related Reading
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
IdeS enzyme is used as a tool enzyme in the preparation and structural characterization analysis of antibody drugs. IdeS enzyme can also assist in drug development in certain fields.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
- Cheng Y, Shi D, Ye R, et al. Noninvasive evaluation of PD-L1 expression in non-small cell lung cancer by immunoPET imaging using an acylating agent-modified antibody fragment. Eur J Nucl Med Mol Imaging. 2023;50(6):1585-1596. doi:10.1007/s00259-023-06130-6(IF:9.1)
- Cheng Y, Shi D, Xu Z, et al. 124I-Labeled Monoclonal Antibody and Fragment for the Noninvasive Evaluation of Tumor PD-L1 Expression In Vivo [published online ahead of print, 2022 Mar 4]. Mol Pharm. 2022;10.1021/acs.molpharmaceut.2c00084. doi:10.1021/acs.molpharmaceut.2c00084(IF:4.939)
- Related Reading
- IdeS Protease：High Specificity IgG Degrading Enzyme
