| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human IFI44L recombinant protein (Position: R16-I452) was used as the immunogen for the IFI44L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IFI44L Antibody / Interferon-induced protein 44-like is a anti-IFI44L Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: IFI44L
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, FACS, ELISA
Biological background
IFI44L is encoded by the IFI44L gene on human chromosome 1p31.1. The protein is approximately 444 amino acids in length and localizes primarily in the cytoplasm, with some nuclear presence following interferon stimulation. Although its exact biochemical function remains under investigation, IFI44L is known to interact with cytoskeletal and RNA-binding proteins, influencing viral RNA recognition and replication.
The IFI44L antibody detects a 49 kilodalton protein by western blot and shows diffuse cytoplasmic staining under immunofluorescence microscopy. IFI44L expression is tightly correlated with type I interferon activity, serving as a biomarker for systemic lupus erythematosus and other interferonopathies. It enhances the antiviral state by restricting replication of RNA viruses such as influenza, hepatitis C, and coronaviruses through modulation of double-stranded RNA signaling and translation inhibition.
In addition to antiviral roles, IFI44L modulates immune cell differentiation and inflammation. Elevated IFI44L levels contribute to interferon signature profiles observed in autoimmune diseases, whereas reduced expression can impair viral clearance. Epigenetic regulation of the IFI44L promoter influences susceptibility to immune dysregulation and chronic infection.
Because IFI44L serves as a downstream effector of interferon signaling, it provides a useful marker for evaluating innate immune activation and viral suppression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
