IFKine™ Orange Donkey Anti-Mouse IgG

Reactivity: Mouse

What it means: This secondary antibody is designed to recognize mouse primary antibodies (e.g., mouse IgG). It binds to the primary antibody and enables detection through its conjugate (enzyme or fluorophore).

Why it matters: Choose “Mouse” reactivity when your primary antibody is raised in mouse. Note that in mouse-derived samples, endogenous mouse immunoglobulins may increase background in certain applications.

Host: Donkey

What it means: “Host” refers to the animal in which the secondary antibody was produced. Host: Donkey means the antibody was generated by immunizing a donkey, and the resulting donkey antibodies were collected and purified to make this secondary antibody.

Why it matters: The host species can affect background and cross-reactivity. For best results, avoid using a donkey-host secondary in workflows where donkey immunoglobulins are present (e.g., donkey serum-based blocking, donkey primary antibodies, or donkey-derived samples), unless the protocol includes appropriate blocking and controls.

Application(s): IF, ICC, FCM

IF (Immunofluorescence): Used as a secondary antibody to bind the primary antibody and provide a fluorescent signal for target visualization under a fluorescence microscope (depending on the conjugate and filter set).

ICC (Immunocytochemistry): Used as a secondary antibody to detect primary antibodies bound to cellular targets in fixed cells, enabling fluorescent imaging of protein localization with the appropriate conjugate.

FCM (Flow Cytometry): Used as a secondary antibody to label primary antibody–bound cells for fluorescence-based detection and analysis by flow cytometry (matched to the instrument’s laser and emission channels).

Isotype: Donkey IgG

What it means: This secondary antibody is a donkey-derived immunoglobulin G (IgG). In other words, it is an IgG antibody produced in donkey and purified for use as a secondary reagent.

Why it matters: “Donkey IgG” is mainly useful for understanding the species origin and antibody class of the secondary antibody:

• Species origin (donkey): This tells you the secondary itself is made in donkey. If your assay uses donkey serum for blocking, or your sample contains donkey immunoglobulins, you may need to optimize blocking or choose a different host secondary to minimize background.
• Antibody class (IgG): IgG is the most common antibody class used for secondary antibodies and is well-suited for routine detection due to strong binding and consistent performance.
• Detection and capture compatibility: Many detection systems and reagents (e.g., anti-donkey IgG reagents, and in some workflows Protein A/G) are designed to work with IgG-class antibodies. Knowing it is donkey IgG helps confirm compatibility when additional binding/capture steps are involved.
• Controls: If you are using anti-donkey detection reagents, “donkey IgG” helps when selecting appropriate negative controls to evaluate non-specific binding from donkey-derived antibodies.