| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
IGF1 (insulin-like growth factor 1), also known as somatomedin-C, is a peptide hormone with strong structural homology to insulin. Produced mainly by the liver in response to growth hormone, and also locally in many tissues, IGF1 signals through the IGF1 receptor tyrosine kinase to activate the PI3K-AKT and RAS-MAPK pathways. This signaling promotes cell proliferation, growth, survival, and differentiation, and IGF1 is a key mediator of postnatal somatic growth and tissue regeneration. Dysregulated IGF1 signaling is associated with growth disorders, metabolic disease, and cancer progression, making IGF1 an important target in endocrinology, metabolism, and oncology research.
Product Description & Applications
The IGF1A ORF cDNA Lentivirus enables stable overexpression of the human insulin-like growth factor 1 (IGF1A, transcript variant 4) open reading frame in mammalian cells. A CMV or EF1a promoter drives the IGF1A ORF fused to a C-terminal V5 tag, with a fluorescent reporter (GFP or RFP) and antibiotic selection marker separated by a self-cleaving peptide for independent translation from a single transcript. The optimized third-generation vector provides high gene expression and reliable genome integration for rapid stable cell line generation. Applications include studying IGF1 signaling, cell growth and proliferation, metabolism, and IGF pathway pharmacology. Particles transduce difficult-to-transfect cells, including primary and thawed cells; cloning is sequence-verified and protein expression validated by transient transfection.
About This Product
This ORF cDNA lentivirus enables stable overexpression of IGF1 (NCBI Accession: NM_000618) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
