| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ig gamma 1 chain C region; IGHG1; Immunoglobulin Gm1; |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IgG1. |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes IgG1. IgG1 polyclonal antibody detects endogenous levels of IgG1 protein.
Validated Applications
| Applications | Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:500-1:1000 |
| IHC | 1:100-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IgG1.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IgG1. |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 41 kDa |
| Swiss-Prot | P01857 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for IgG1 research from Bioworld Technology Inc, or contact us for alternative formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
