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The IGR-1 cell line is derived from a human malignant melanoma, making it a valuable model for studying the pathophysiology of melanoma and testing anti-cancer therapies. These cells are epithelial in nature and exhibit characteristics typical of aggressive melanoma, including rapid proliferation and the ability to form colonies in soft agar, a hallmark of oncogenic transformation. The IGR-1 cell line is particularly useful in research focused on understanding the molecular mechanisms driving melanoma progression, as well as in the development and testing of targeted therapies and immunotherapies.
IGR-1 cells harbor mutations common in melanoma, including alterations in the MAPK/ERK pathway, which is often dysregulated in this cancer type. These mutations contribute to the cell line's ability to proliferate uncontrollably and resist apoptosis. Researchers utilize IGR-1 cells to investigate the effects of various inhibitors on this signaling pathway, providing insights into potential therapeutic strategies. Additionally, the cell line's expression of melanoma-associated antigens makes it suitable for studying immune responses against melanoma, including the development of novel immunotherapeutic approaches.
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- Tumorigenic: Yes, in nude mice.
- Products: Melanin
- Mutational profile: IGR-1 cells carry a heterozygous BRAFV600K mutation, but they are wild type with respect to BRAFV600E.
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 3 x 104/cm2 after thawing, 1 to 2 x 104/cm2 for routine splitting
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: 1 to 2 days
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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