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The IGROV-1 cell line is a human ovarian adenocarcinoma cell line extensively utilized in research, particularly in studies involving ovarian cancer. Derived from an ovarian carcinoma, IGROV-1 cells are known for their utility in modeling epithelial ovarian cancer (EOC), which accounts for a majority of ovarian malignancies. This cell line has been employed in various contexts, including evaluating drug responses and mechanisms underlying drug resistance. For example, IGROV-1 has been instrumental in testing the efficacy of targeted therapies, such as the folate receptor alpha-targeting antibody-drug conjugate mirvetuximab soravtansine (IMGN853). This ADC showed promising results by synergizing with chemotherapeutics like carboplatin and doxorubicin, enhancing antitumor efficacy through DNA damage and cell cycle arrest in preclinical models.
In addition to its role in cancer research, IGROV-1 has been characterized as a model for viral infection studies. Recent work highlighted its susceptibility to SARS-CoV-2, leveraging its expression of ACE2 to support viral replication. IGROV-1 was shown to mount a robust innate immune response upon infection, similar to primary human nasal epithelial cells, indicating its potential for serological assays, antiviral drug testing, and isolation of viral variants from patient samples. This cell line is considered advantageous for research due to its effective replication of viruses compared to traditional models like Vero cells, which can lead to adaptive mutations.
Overall, IGROV-1 cells serve as a valuable model in both oncology and virology, supporting studies of tumor biology, drug resistance, and viral pathogenesis. Their relevance in drug synergy experiments and their compatibility with antiviral research underscore their versatility and importance in the field.
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- Tumorigenic: Yes, in nude mice.
- Mutational profile: Mutation: BRCA1, p.Lys654Serfs*47 (c.1961delA), heterozygous; Mutation: BRCA2, p.Lys1108Argfs*11 (c.3323delA) (p.Gln1107fs) (c.3320delA); Mutation: PIK3CA, p.Arg38Cys (c.112C>T), heterozygous; Mutation: PIK3CA, p.Ter1069TrpinsLysAspAsn (c.3207A>G), heterozygous; Mutation: PTEN, p.Thr319fs*1 (c.955_958delACTT) (p.VL317fs) (V317fs*3), heterozygous; Mutation: RB1, p.Val654Cysfs*4 (c.1959delA), heterozygous; Mutation: SMAD4, p.Gly231Alafs*10 (c.692delG), heterozygous; Mutation: SMAD4, p.Leu495Pro (c.1484T>C), heterozygous; Mutation: TP53, p.Ser90Leufs*59 (c.267dupC) (c.267_268insC), heterozygous; Mutation: TP53, p.Tyr126Cys (c.377A>G), heterozygous
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with TrypLE Express, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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