IKCA1 (KCNN4) HEK293 Cell Line

What it means: The parental cell line is the host in which the transgene or reporter was stably integrated. HEK293 (Human Embryonic Kidney 293) is a epithelial-like, adherent cell line selected for this product.

Why it matters:

• Enables high transfection efficiency and robust transgene expression; widely used for receptor pharmacology, ion channels, and reporter gene assays.
• The parental background determines endogenous receptor expression, growth properties, and media requirements—verify compatibility with your assay conditions.
• Secondary reagents (antibodies, growth media) should be validated for this host species and cell type.

How to interpret here: This product is engineered on a HEK293 background. Pathway activity readouts, IC50/EC50 values, and control responses are calibrated for this host. Results may differ in alternative cell backgrounds.

What it means: Species refers to the biological origin of the expressed gene or protein target in this cell line. This product is classified as Human origin.

Why it matters:

• The transgene or expressed protein is of human sequence. This maximizes compatibility with human-specific antibodies, human interactome databases, and clinically relevant pathway contexts.
• Species origin directly affects antibody cross-reactivity, functional assay interpretation, and translational relevance of results.
• Confirm that your detection antibodies, assay controls, and signaling reagents are validated for Human targets.

How to interpret here: The Human designation indicates the sequence and biological context of the expressed construct—not necessarily the host cell species. Check the host cell line chip for the cellular background.

What it means: BSL-2 (Biosafety Level 2) indicates this material poses moderate risk and requires specific containment practices and training.

Why it matters:

• Work must be performed in a certified BSL-2 laboratory; access is restricted to trained personnel.
• All procedures generating aerosols or splashes must be conducted in a Class II Biological Safety Cabinet (BSC).
• PPE required: lab coat, gloves, and eye/face protection. Decontaminate all waste before disposal.
• Institutional Biosafety Committee (IBC) approval is required prior to use.

How to interpret here: BSL-2 classification means your facility must be BSL-2 certified and your team trained for BSL-2 handling before working with this product. Confirm current IBC approval status and maintain a decontamination log.

Tip: If you are uncertain whether your laboratory is BSL-2 certified, contact your institutional Environmental Health & Safety (EH&S) office.

What it means: Recombinant Cell Line. A Recombinant Cell Line stably expresses a heterologous gene of interest in the host cell background at defined levels confirmed by molecular characterization.

Why it matters:

• Stable integration ensures reproducible target expression across passages, eliminating the variability of transient transfection experiments.
• Select the correct product type based on whether your experiment requires overexpression, loss-of-function, pathway reporting, or immune effector function.

How to interpret here: This Recombinant Cell Line expresses the target protein indicated in the product title. Verify protein expression by Western blot or functional assay upon receipt and after extended culture.

What it means: Confirmed Negative indicates this cell line was screened using a validated mycoplasma detection assay prior to release and confirmed negative for mycoplasma contamination.

Why it matters:

• Mycoplasma contamination can significantly alter gene expression profiles, proliferation rates, metabolism, and assay readouts—potentially invalidating experimental results without visible signs of contamination.
• Testing prior to use is considered best practice in accordance with ATCC and ECACC guidelines for cell line authentication.
• BPS Bioscience uses validated PCR-based or luminescence-based detection kits (e.g., Venor™GeM, MycoAlert™) for screening.

How to interpret here: Confirmed Negative confirms the product shipped to you passed mycoplasma screening at the time of manufacture. We still recommend performing routine mycoplasma testing in your laboratory after receipt and during extended culture to confirm ongoing negative status.

Tip: Test your cell cultures for mycoplasma at regular intervals (e.g., every 2–4 weeks) regardless of the vendor QC status. Contamination can be introduced during routine culture handling.

What it means: Each vial contains ~2 X 10^6 cells in 1 indicates the manufacturer-recommended storage condition for maintaining this product's viability and activity.

Why it matters:

• Incorrect storage temperature accelerates degradation and can result in reduced viability, altered expression, or complete loss of function.
• Follow storage instructions exactly as stated to maintain product performance within the specified shelf life.

How to interpret here: Store this product at Each vial contains ~2 X 10^6 cells in 1 as directed. Refer to the product datasheet for complete handling guidelines and shelf-life information.

What it means: The biological activity describes the functional application or validated use of this cell line as characterized by the manufacturer. Key activity: N-terminal FLAG-tagged human IKCA1 has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting.

Why it matters:

• Confirms the cell line performs as intended for the specified pathway or assay type—this is the primary use case the product was engineered and validated for.
• Using the cell line for assays outside the stated biological activity may require additional validation on your end.

How to interpret here: This describes the assay context in which this cell line has been validated. Align your experimental design with the stated biological activity for optimal results. Consult the product datasheet for validated stimuli, inhibitors, and dose-response reference data.