IKK Alpha/Beta Polyclonal Antibody

SKU:BHA12105689
Overview
Click light‑blue chips for details
CHUK-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, ELISA in Human, Mouse, Rat samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
Molecular Weight ~84.7/~86.6 kDa
UniProt O15111
Gene ID Human: 1147/3551; Mouse: 16150; Rat: 84351
Applications 3 validated
Available Options
Size
Options selector
Catalog no. Size
BT-AP04421-20UL 20 uL
BT-AP04421-50UL 50 uL
BT-AP04421-100UL 100 uL
Field Specification
Alternative Names CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B ki
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 1147/3551; Mouse: 16150; Rat: 84351
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
Isotype
  • IgG
Molecular Weight ~84.7/~86.6 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Storage -20°C for 1 year
Target CHUK

Product Overview

Primary Antibody

This antibody targets CHUK and detects endogenous levels of CHUK protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target CHUK
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Molecular Weight ~84.7/~86.6 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 1147/3551; Mouse: 16150; Rat: 84351
UniProt O15111 (Human: O15111/O14920; Rat: Q9QY78)

Scientific Background

CHUK encodes a member of the serine/threonine protein kinase family. Inhibitor of nuclear factor kappa-B kinase subunit alpha, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Alternative Names

CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B ki

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetCHUK
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Molecular Weight~84.7/~86.6 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human IKK-alpha/beta. AA range:141-190
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 1147/3551; Mouse: 16150; Rat: 84351
UniProtO15111 (Human: O15111/O14920; Rat: Q9QY78)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

CHUK encodes a member of the serine/threonine protein kinase family. Inhibitor of nuclear factor kappa-B kinase subunit alpha, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Alternative Names

CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B ki

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of CHUK?
The predicted molecular weight of CHUK is ~84.7/~86.6 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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