| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
Interleukin-2 (IL-2) is a key cytokine produced by activated T cells that drives T cell proliferation, effector differentiation, and regulatory T cell homeostasis. Transcription of the IL-2 gene is induced following T cell receptor and co-stimulatory signaling and depends on a compact promoter that integrates inputs from transcription factors including NFAT, NF-κB, and AP-1. NFAT activation, downstream of calcium signaling and calcineurin, is a central driver of IL-2 promoter activity. Because IL-2 induction is a hallmark of T cell activation, IL-2 promoter activity is a widely used readout in immunology, inflammation, and T cell biology research.
Product Description & Applications
The IL-2 Promoter Reporter Lentivirus provides a sensitive fluorescent or luminescent readout of T cell receptor-driven transcription. The construct combines a ZIP-NFAT element with the minimal human IL-2 promoter region upstream of a reporter gene, offering a range of fluorescent and luminescent options including EGFP, mCherry, BFP2, d2GFP, firefly luciferase, Gaussia luciferase, and Renilla luciferase. A constitutively expressed selection marker (blasticidin, hygromycin, puromycin, or zeocin) supports stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.
It is suited to studies of T cell activation, cytokine signaling, and pathway modulation, and to drug screening.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the IL-2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
