IL-2R Alpha Polyclonal Antibody

SKU:BHA12105754
Overview
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Anti-IL2RA antibody (Rabbit, polyclonal, IgG, unconjugated). Validated for Western Blot, Immunofluorescence, ELISA. Reacts with Human. Suitable for routine research immunoassays. Supplied in liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality Polyclonal
Host Rabbit
Reactivity Human
Applications Western Blot, Immunofluorescence, ELISA
Conjugate Unconjugated
Target MW 30819 Da
UniProt Human: P01589; Mouse: P01590
Options selector
Catalog no. Size
BT-AP04486-20UL 20 uL
BT-AP04486-50UL 50 uL
BT-AP04486-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 20 uL, 50 uL, 100 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -20°C for 1 year
  • Shipping: Please contact us to confirm shipping conditions for this product.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target IL2RA
Alternative names IL2RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD antigen CD25
Gene ID Human: 3559
UniProt # Human: P01589; Mouse: P01590
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25. AA range:223-272
Conjugate
  • Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Catalog no. (Mfr.) BT-AP04486
Main SKU BHA12105754
Polyclonal Antibody

Scientific Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Product Overview

This polyclonal antibody, raised in Rabbit, recognizes IL2RA. It is validated for Western Blot, Immunofluorescence, ELISA.

Validated Applications

Applications Western Blot, Immunofluorescence, ELISA

Optimal dilutions should be determined by the end user for each application and sample type.

Immunogen

The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25. AA range:223-272

Specifications

Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human
Immunogen The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25. AA range:223-272
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Target MW 30819 Da
UniProt Accession Human: P01589; Mouse: P01590
Gene ID Human: 3559
Storage -20°C for 1 year
Lead Time Within one week

Reactivity Notes

Validated reactivity: Human. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.

Safety & Handling

This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.

Related Products

Explore related antibodies and reagents for IL2RA research from Bioassay Technology Laboratory, or contact us for alternative formats.

Q.Which applications is this antibody validated for?
A.Validated for Western Blot, Immunofluorescence, ELISA. Determine optimal dilutions empirically.
Q.What are the host, clonality, and isotype?
A.Raised in Rabbit as a polyclonal antibody, isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen..
Q.What was the immunogen?
A.The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25. AA range:223-272
Q.Which species does it react with?
A.Human. Validate cross-reactivity for unlisted species with appropriate controls.
Q.How should I store and handle this antibody?
A.-20°C for 1 year Storage buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.. Shipped with cold-chain packaging. RUO - not for diagnostic or therapeutic use.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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