| Field | Specification |
|---|---|
| Target | |
| Alternative names | CLMF; CLMF p35; CLMF p40; CLMF1; CLMF2; Cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 2; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12A; IL 2 subunit p40; IL-12 subunit p35; IL-12A; IL-12B; IL12; IL12, p35 subunit; IL12, subunit p40; IL12A; IL12A_HUMAN; IL12B; IL35 subunit; Interleukin 12 alpha chain; Interleukin 12 beta chain; Interleukin 12 subunit beta; Interleukin 12, p40; Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); Interleukin 12B; Interleukin-12 subunit alpha; Natural killer cell stimulatory factor 1, 35 kD subunit; natural killer cell stimulatory factor 2; Natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Interleukin-12 subunit alpha protein (23-219AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-IL12A antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect IL12A in Human for immunology research.
Key elements and design rationale
- Immunogen: Recombinant Human Interleukin-12 subunit alpha protein (23-219AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
Biological background
IL12A (also: CLMF, CLMF p35, CLMF p40, CLMF1, CLMF2, Cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 2, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12A, IL 2 subunit p40, IL-12 subunit p35, IL-12A, IL-12B, IL12, IL12, p35 subunit, IL12, subunit p40, IL12A, IL12A_HUMAN, IL12B, IL35 subunit, Interleukin 12 alpha chain, Interleukin 12 beta chain, Interleukin 12 subunit beta, Interleukin 12, p40, Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35), Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), Interleukin 12B, Interleukin-12 subunit alpha, Natural killer cell stimulatory factor 1, 35 kD subunit, natural killer cell stimulatory factor 2, Natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NFSK, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF, NKSF1, NKSF2, P35) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current IL12A annotation relevant to immunology.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
IL12A is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects IL12A in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
HRP: direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing IL12A; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
