| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Promoter | |
| Selection Marker | Puromycin |
| Shipping | |
| Species |
Background
IL2RB (interleukin 2 receptor subunit beta, also called CD122) is a shared signaling component of the receptors for interleukin-2 and interleukin-15. Together with the common gamma chain and a cytokine-specific alpha subunit, IL2RB forms high- and intermediate-affinity receptor complexes that transmit signals through JAK1 and JAK3 to activate STAT5 and downstream pathways. This signaling controls the proliferation, survival, and differentiation of T cells, natural killer cells, and regulatory T cells, making IL2RB central to immune homeostasis. Dysregulated IL-2 and IL-15 signaling is implicated in autoimmunity, immunodeficiency, and cancer, and IL2RB is of interest for engineered cytokine and immunotherapy approaches.
Product Description & Applications
The IL2RB ORF cDNA Lentivirus delivers the full open reading frame of human IL2RB for stable overexpression in mammalian cells. Expression is driven by a CMV promoter, and constructs may include a C-terminal epitope tag, a fluorescent reporter, and a puromycin selection marker, with tag, reporter, and marker elements linked by self-cleaving peptide sequences to allow independent protein production.
Supplied as high-titer, third-generation, VSV-G-pseudotyped particles in serum-free RPMI-1640, the lentivirus efficiently transduces primary, thawed, and other difficult-to-transfect cells. Applications include gain-of-function studies of IL-2 and IL-15 receptor signaling, generation of stable overexpression cell lines, and reconstitution experiments in immunology and cancer research, supporting both in vitro and in vivo work.
About This Product
This ORF cDNA lentivirus enables stable overexpression of IL2RB (NCBI Accession: NM_000878.5) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP or RFP; Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.
Ultra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
