| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human CD125 was used as the immunogen for the IL5RA antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
IL5RA Antibody / CD125 is a anti-IL5RA Rabbit antibody Recombinant Rabbit Monoclonal clone 31I14 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: IL5RA
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31I14, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
IL5RA antibody is widely applied in immunology, allergy research, and hematology. Expression of IL5RA is restricted to eosinophils, basophils, and certain progenitor cells, making it a valuable marker of eosinophilic inflammation. By detecting IL5RA, researchers can evaluate eosinophil biology in conditions such as asthma, eosinophilic esophagitis, and hypereosinophilic syndromes.
Flow cytometry with IL5RA antibody distinguishes eosinophil populations in blood and tissue. Immunohistochemistry maps receptor expression in inflamed airway or gastrointestinal biopsies, while western blotting confirms protein expression in hematopoietic samples. These techniques provide robust tools for studying IL5RA expression and signaling.
IL5RA is a therapeutic target in eosinophilic diseases. Monoclonal antibodies such as benralizumab target IL5RA to deplete eosinophils by antibody dependent cytotoxicity. By applying IL5RA antibody, researchers can monitor receptor levels, assess drug effects, and explore mechanisms of eosinophil regulation.
Beyond allergy and hematology, IL5RA contributes to host defense against parasites by supporting eosinophil mediated immunity. Dysregulation leads to pathological inflammation in chronic asthma and gastrointestinal disorders. IL5RA antibody therefore provides a critical reagent for understanding type 2 immune responses in health and disease.
IL5RA antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
