| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The immortalized HK/FDC cell line is a genetically stabilized derivative of the original HK follicular dendritic cell-like cells, retaining key phenotypic and functional characteristics while enabling extended propagation without the senescence-associated limitations of the parental culture. Immortalization was achieved through the introduction of defined genetic elements that bypass replicative arrest, facilitating consistent long-term studies of germinal center biology and FDC–B cell interactions. Immortalized HK/FDC cells maintain the capacity to bind and co-stimulate germinal center B cells, promote their survival, and enhance their proliferation in the presence of signals such as anti-IgM or CD40 ligation. Importantly, they continue to express adhesion molecules and costimulatory factors characteristic of FDCs, including VCAM-1 and ICAM-1, and secrete soluble mediators that mimic the microenvironmental support provided by native FDCs. These properties make the immortalized HK/FDC line a robust and reproducible model for dissecting the cellular and molecular mechanisms governing B cell maturation, affinity selection, and survival within the germinal center.
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Viruses: Cytion, immortalized by Inscreenex i.A.
- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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