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| Product format | Frozen |
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Overview
The Immortalized Human Dopaminergic Neuronal Precursor Cells, also known as the Lund Human Mesencephalic (LUHMES) cell line, is a subclone of the tetracycline-controlled, v-myc-overexpressing human mesencephalic-derived cell line MESC2.10. This cell line is unique in that it can be differentiated to acquire a dopaminergic neuron-like phenotype under appropriate growth conditions.
Key elements and design rationale
- Model identity: Immortalized Human Dopaminergic Neuronal Precursor Cells (LUHMES) is supplied as an immortalized cell line derived from Human brain.
- Growth properties: Adherent, flat dendritic processes
- Growth conditions: Grow cells in culture vessels* pre-coated with 50 μg/ml poly-L-ornithine (PLO) (TM062) and 1 μg/ml fibronectin (TM059) in H₂O for at least 3 hours at 37°C. After 3-hour incubation, aspirate the coating solution, rinse vessel with sterile H₂O or PBS 3 times, and use the vessel right away.*Do not grow cells in culture vessels with surface areas equal to or less than 12.5 cm²; cells do not grow in 6-well, 24-well, 48-well, or 96-well plates. Advanced DMEM/F12 (Gibco;12634010) + 1X N2 supplement (ThermoFisher Scientific) + 2 mM L-Glutamine (G275) + 40 ng/ml rh FGF2 (Z101455) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Do not let media colour change to orange-yellow. Note: cells are sensitive to freeze-thaw conditions and take additional time to recover. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.
- Product format: Frozen, BSL-2
This cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor/background information is available for contextual interpretation.
Biological background
LUHMES expresses functional dopamine transporter (DAT), vesicular monoamine transporter (VMAT-2), tyrosine hydroxylase (TH) and the neuronal form of β-III tubulin after differentiation. In addition to retaining dopaminergic neuronal-specific markers, LUHMES also exhibit electrophysiological properties, thus making this cell line a valuable neuronal model for neurodevelopmental studies, disease modelling and neuropharmacology. May take 4 to 5 days for cells to recover in culture. Donor/background information provided for this product: 8-week-old fetal human ventral mesencephalon.
Research relevance and current trends
- Cultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.
- Researchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.
- Interpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.
Common research applications
- Neurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.
- Cell-based assays that compare experimental perturbations across defined media and substrate conditions.
- Phenotype tracking using morphology, marker expression, or reporter output where applicable.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: Grow cells in culture vessels* pre-coated with 50 μg/ml poly-L-ornithine (PLO) (TM062) and 1 μg/ml fibronectin (TM059) in H₂O for at least 3 hours at 37°C. After 3-hour incubation, aspirate the coating solution, rinse vessel with sterile H₂O or PBS 3 times, and use the vessel right away.*Do not grow cells in culture vessels with surface areas equal to or less than 12.5 cm²; cells do not grow in 6-well, 24-well, 48-well, or 96-well plates. Advanced DMEM/F12 (Gibco;12634010) + 1X N2 supplement (ThermoFisher Scientific) + 2 mM L-Glutamine (G275) + 40 ng/ml rh FGF2 (Z101455) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Do not let media colour change to orange-yellow. Note: cells are sensitive to freeze-thaw conditions and take additional time to recover. For information regarding cell differentiation, please refer to the Differentiation Protocol PDF under the Documents Tab.
- Seeding Density (cells/cm²): 50,000 - 100,000
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media supplemented with 10% FBS. Centrifuge cells at 125xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Cells take 4-5 days to recover and attach. Do not change media during this time. Add additional fresh growth media as needed.
- Perform media changes regularly; do not let media change colour to orange-yellow.
- Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media supplemented with 10% FBS into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
