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| Product format | Frozen |
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Overview
Dermal papilla (DP) cells are specialized mesenchymal cells located at the base of hair follicles. They play a crucial role in hair growth and maintenance by interacting with surrounding cells, including keratinocytes and melanocytes.
Key elements and design rationale
- Model identity: Immortalized Human Frontal Scalp Dermal Papilla Cells (F Clone 2) is supplied as an immortalized cell line derived from Human hair follicle.
- Growth properties: Adherent, fibroblast-like
- Growth conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow III (TM003) + 10% HI-FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 5 µg/ml Hygromycin B (G265), and 5 µg/ml Geneticin/G418 (G271) Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
- Product format: Frozen, BSL-2
This cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor/background information is available for contextual interpretation.
Biological background
Cultured DP cells have been widely utilized in hair growth and regeneration studies. However, they undergo significant morphological changes in vitro and progressively lose their characteristic features. Moreover, prolonged culturing leads to a decline in their growth rate and overall proliferative capacity. Androgenetic alopecia (AGA), commonly known as male pattern baldness, is a hereditary condition that primarily affects scalp hair. It is characterized by the progressive miniaturization of hair follicles, resulting in thinner, shorter, and less pigmented hair, ultimately leading to hair loss. Unlike DP cells in the occipital (O) region, DP cells in the frontal (F) region of individuals with AGA are androgen-dependent, specifically responding to dihydrotestosterone (DHT), a potent form of testosterone. F-region DP cells are more sensitive to DHT than O-region DP cells, making frontal hair follicles more prone to miniaturization and eventual hair loss. Until now, the availability of DP cell lines from both O and F regions of the same patients has been a long-standing challenge in hair growth research and therapeutic development. For the first time in history, abm has made these invaluable DP cell lines commercially accessible to scientists in both academia and biotech industries, offering a groundbreaking tool for studying hair follicle biology and developing novel treatments for AGA. Donor/background information provided for this product: Male, 42, Androgenetic alopecia.
Research relevance and current trends
- Cultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.
- Researchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.
- Interpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.
Common research applications
- Expansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.
- Comparative experiments that examine changes in morphology, marker expression, or functional readouts over time.
- Assay development in culture systems where media composition and substrate selection influence interpretation.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) PriGrow III (TM003) + 10% HI-FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 5 µg/ml Hygromycin B (G265), and 5 µg/ml Geneticin/G418 (G271) Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
- 3D Culture Conditions: Procedure Preparation (before 3D Culture): Thaw cells following "Thawing Protocol" and "Growth Conditions". Expand cells for one passage, then seed for spheroid generation (3D culture). Subculture Protocol:Aspirate the supernatant and wash cells with 1X PBS (pH 7.4) (G5000). Dissociate cells using 1–1.5 ml of Gentle Dissociation Solution (TM080). Observe the cells under a microscope to confirm detachment (typically within 2–10 minutes). Cells that are difficult to detach can be put in 37 °C for several minutes to facilitate detachment.Neutralize using an equal volume of complete growth media (must contain serum) or using Trypsin Neutralizing Solution (TM069).Transfer culture suspension into sterile conical centrifuge tube (G5500), and centrifuge at 125 x g for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.Aspirate supernatant and re-suspend the pellet with pre-warmed fresh complete growth media for 3D Culture. Spheroid Generation Protocol:Count cells and viability using cell counter or hemocytometer with Trypan Blue Stain (TM071). Proceed if cells are >90% viability.Seed 500 to 10,000 cells per well in SpheroWell™ 96 Well Plate (G7540), final volume maintained at 100 μl per well. Avoid cell clumps (clumps will not generate uniform spheroids). Count as Day 0. Tip: Fill the outermost wells of the plate with 1X PBS (pH 7.4) (G5000) to prevent evaporation of media during incubation.(Recommended) Centrifuge the sealed plate at 300 x g for 5 minutes.Place in the incubator set at 37 °C, 5% CO₂. Change Media:Every 2–3 days, add 100 μl of fresh media slowly along the wall of the well to avoid disrupting spheroids at the bottom.Take out 100 μl of media slowly and discard from the well.(Recommended, but optional) Centrifuge the sealed plate at 300 x g for 5 minutes. Monitor the Cells for 3D/Spheroid Formation:Spheroid formation will appear between day 5 and 10.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed Gentle Dissociation Solution (TM080) to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize the Gentle Dissociation Solution (TM080) by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
