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| Product format | Frozen |
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Overview
These Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) are a robust model for investigating osteoarthritis (OA) pathogenesis and developing therapeutric interventions for OA. Derived from human articular cartilage of osteoarthritis patients, these cells are immortalized to enhance longevity and stability.
Key elements and design rationale
- Model identity: Immortalized Human Osteoarthritis Cartilage Mesenchymal Stem Cells (OASC5) is supplied as an immortalized cell line derived from Human cartilage.
- Growth properties: Adherent, fibroblast-like
- Growth conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS + 100mM HEPES (TM058) + 2mM L-Glutamine (G275) + 0.1 mM Ascorbic acid + 0.1mM Sodium Pyruvate (TM057) + 2.7μM L-glucose + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. For information regarding cell differentiation, please refer to the "Differentiation Protocol" PDF under the Documents Tab.
- Product format: Frozen, BSL-2
This cell-based model is generally used in cell fate, differentiation, and expansion studies. Donor/background information is available for contextual interpretation.
Biological background
Thus enabling extensive experimental manipulation without the typical constraints of primary cell cultures. OASC5 cells display a unique stellate morphology, intermediate between chondrocytes and bone-marrow derived mesenchymal stem cells. The cells express MSC markers such as CD166, CD105, and CD90 under specific conditions, affirming their identity as mesenchymal stem cells and are capable of differentiating into chondrocytes, osteoblasts, and adipocytes, offering a versatile tool for studying various aspects of cell differentiation. Donor/background information provided for this product: Adult, Pooled male (61) and female (69), Osteoarthritic articular cartilage.
Research relevance and current trends
- Cultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.
- Researchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.
- Interpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.
Common research applications
- Expansion and maintenance studies under defined growth conditions prior to downstream differentiation or phenotype analysis.
- Comparative experiments that examine changes in morphology, marker expression, or functional readouts over time.
- Assay development in culture systems where media composition and substrate selection influence interpretation.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow III (TM003) + 10% FBS + 100mM HEPES (TM058) + 2mM L-Glutamine (G275) + 0.1 mM Ascorbic acid + 0.1mM Sodium Pyruvate (TM057) + 2.7μM L-glucose + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. For information regarding cell differentiation, please refer to the "Differentiation Protocol" PDF under the Documents Tab.
- Split Ratio: 1:3
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 120xg for 2 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
- Aspirate the culture media, and add 2-3ml of pre-warmed Gentle Dissociation Solution (TM080) to the culture vessel.
- Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.
- Neutralize the Gentle Dissociation Solution (TM080) by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
