| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Tissue | |
| Growth properties | |
| Biosafety level | |
| Product format | Frozen |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
As messengers bridging the innate and adaptive immune systems, conventional/classical lymphoid tissue-resident dendritic cells (cDCs) capture and present antigens; acting as sentinels in secondary lymphoid organs and other tissues. The wild-type MutuDC1940 dendritic cell line is derived from mouse spleen tissues (CD11c:SV40LgT transgenic mice) and is a powerful tool for vaccine science and immunotherapy, particularly for strategizing target antigens to the CD8α+ subset (which has the CD11chigh, B220−, DEC205+, CD24high, CD11b− expression profile).
Key elements and design rationale
- Model identity: Immortalized Mouse Dendritic Cells (MutuDC1940) is supplied as an engineered cell line derived from Mouse spleen.
- Growth properties: Adherent, small aggregates
- Growth conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 4 mM Glutamax + heat-inactivated 10% FBS + 1% of 7.5% Sodium Bicarbonate Solution + 50 µM β-mercaptoethanol + 10 mM HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Filter the complete media with a 0.22µm filter prior to use.Note: cells must be seeded and maintained at high density for optimal growth. Cells are sensitive to culture conditions; change the complete media every 2-3 days. Do not let media colour change to yellow.
- Engineering / immortalization: Gfp reporter expression.
- Product format: Frozen, BSL-2
This cell-based model is generally used in immunology, hematology, and signaling studies. Donor/background information is available for contextual interpretation.
Biological background
MutuDC cell lines are GFP positive due to the GFP reporter in the CD11c:SV40LgT transgene. T3034 - TLR3 Knockout MutuDC Cell line Cat. T3035 - TLR9 Knockout MutuDC Cell line Cat. T3036 - Ifnar1 Knockout MutuDC Cell line Donor/background information provided for this product: CD11c:SV40LgT-transgenic C57BL/6 mice.
Research relevance and current trends
- Engineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.
- Reporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.
- Expression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.
Common research applications
- Routine expansion and maintenance of a defined cell model for downstream in vitro experiments.
- Phenotype, signaling, or marker-expression studies performed under standardized culture conditions.
- Cell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). PriGrow V (TM015) + 4 mM Glutamax + heat-inactivated 10% FBS + 1% of 7.5% Sodium Bicarbonate Solution + 50 µM β-mercaptoethanol + 10 mM HEPES (TM058) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Filter the complete media with a 0.22µm filter prior to use.Note: cells must be seeded and maintained at high density for optimal growth. Cells are sensitive to culture conditions; change the complete media every 2-3 days. Do not let media colour change to yellow.
- 3D Culture Conditions: Procedure Preparation (before 3D Culture): Thaw cells following "Thawing Protocol" and "Growth Conditions". Expand cells for one passage, then seed for spheroid generation (3D culture). Subculture Protocol:Aspirate the supernatant and wash cells with 1X PBS (pH 7.4) (G5000). Dissociate cells using 1–1.5 ml of Gentle Dissociation Solution (TM080). Observe the cells under a microscope to confirm detachment (typically within 2–10 minutes). Cells that are difficult to detach can be put in 37 °C for several minutes to facilitate detachment.Neutralize using an equal volume of complete growth media (must contain serum) or using Trypsin Neutralizing Solution (TM069).Transfer culture suspension into sterile conical centrifuge tube (G5500), and centrifuge at 125 x g for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.Aspirate supernatant and re-suspend the pellet with pre-warmed fresh complete growth media for 3D Culture. Spheroid Generation Protocol:Count cells and viability using cell counter or hemocytometer with Trypan Blue Stain (TM071). Proceed if cells are >90% viability.Seed 500 to 10,000 cells per well in SpheroWell™ 96 Well Plate (G7540), final volume maintained at 100 μl per well. Avoid cell clumps (clumps will not generate uniform spheroids). Count as Day 0. Tip: Fill the outermost wells of the plate with 1X PBS (pH 7.4) (G5000) to prevent evaporation of media during incubation.(Recommended) Centrifuge the sealed plate at 300 x g for 5 minutes.Place in the incubator set at 37 °C, 5% CO₂. Change Media:Every 2–3 days, add 100 μl of fresh media slowly along the wall of the well to avoid disrupting spheroids at the bottom.Take out 100 μl of media slowly and discard from the well.(Recommended, but optional) Centrifuge the sealed plate at 300 x g for 5 minutes. Monitor the Cells for 3D/Spheroid Formation:Spheroid formation will appear between day 5 and 10.
—
🧊 Thawing Protocol
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 290xg for 5 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
- Incubate the cells at the recommended conditions.
🔬 Subculture Protocol
Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 85-90% confluent. Use a prewarmed (37°C) non-enzymatic, 5 mM EDTA-based cell dissociation buffer (5 mM EDTA in 20 mM Hepes-PBS) to subculture cells.
- Aspirate excess media, gently wash cells with PBS (1X) and add 2-3 ml of the pre-warmed (37°C) disssociation buffer mixture to the culture vessel.
- Incubate culture vessel at 37°C for 3-5 minutes to facilitate detachment. Observe the cells under a microscope to confirm detachment. Gently tap the flask to dislodge any loosely adherent cells.
- Neutralize the solution by adding an equal volume of the complete growth media into the culture vessel.
- Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 290xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
- Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired. Cells must be seeded at high density.
- Incubate the cells at the recommended conditions.
How should I handle live cells once I receive them?
Please refer to our Cell Handling and Thawing Guidelines for detailed instructions on receiving, thawing, and culturing live cells:
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
What is your warranty or return policy?
Our warranty and return policy is outlined in abm’s Terms and Conditions, including details on product quality, limitations, and claims.
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
The number of times cells can divide depends on the cell type:
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Please refer to the Growth Conditions section of your specific product page. This section will indicate whether Applied Cell Extracellular Matrix (G422), PriCoat™ flasks, or both are recommended for optimal cell attachment and growth, as requirements may vary by cell type.
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
10%
OFF
20%
OFF
$50
OFF
FREE
SAMPLE
